ArticleThe Journal of cell biology1993
Commitment to differentiation and expression of early differentiation markers in murine keratinocytes in vitro are regulated independently of extracellular calcium concentrations.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 53 citations in OpenAlex.
- Method and Analysis of High-Calcium Induced Differentiation of Human Primary KeratinocytesMethods and protocols · 2026Article
- Histological subtypes of mouse mammary tumors reveal conserved relationships to human cancers.PLoS genetics · 2018Article
- Rare human papillomavirus 16 E6 variants reveal significant oncogenic potential.Molecular cancer · 2011Article
- EGF receptor signaling blocks aryl hydrocarbon receptor-mediated transcription and cell differentiation in human epidermal keratinocytes.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Human papillomavirus 16 E6 variants differ in their dysregulation of human keratinocyte differentiation and apoptosis.Virology · 2009Article
- Cyclin D1 downregulation is important for permanent cell cycle exit and initiation of differentiation induced by anchorage-deprivation in human keratinocytes.Journal of cellular biochemistry · 2009Article
- Modelling the human epidermis in vitro: tools for basic and applied research.Archives of dermatological research · 2007Review
- Role of integrins in regulating epidermal adhesion, growth and differentiation.The EMBO journal · 2002Review
- Switch from p53 to MDM2 as differentiating human keratinocytes lose their proliferative potential and increase in cellular size.Oncogene · 2000Article
- C/EBPbeta modulates the early events of keratinocyte differentiation involving growth arrest and keratin 1 and keratin 10 expression.Molecular and cellular biology · 1999Article
- Regulation of K3 keratin gene transcription by Sp1 and AP-2 in differentiating rabbit corneal epithelial cells.Molecular and cellular biology · 1997Article
- Calcium inhibits epidermal growth factor-induced activation of p21ras in human primary keratinocytes.Molecular and cellular biology · 1994Article
- Expression and growth inhibitory effect of decapentaplegic Vg-related protein 6: evidence for a regulatory role in keratinocyte differentiation.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Human epidermis reconstructed on synthetic membrane: influence of experimental conditions on terminal differentiation.In vitro cellular & developmental biology. AnimalArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
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Abstract
In the epidermis, one of the earliest characterized events in keratinocyte differentiation is the coordinate induction of a pair of keratins specifically expressed in suprabasal cells, keratin 1 (K1) and keratin 10 (K10). Both in vivo and in vitro, extracellular calcium is necessary for several biochemical and structural changes during keratinocyte differentiation. However, it has been unclear if calcium serves as a differentiation signal in keratinocytes. In these studies, expression of suprabasal keratin mRNA and protein is used to test whether the initial differentiation of primary mouse keratinocytes in vitro is dependent on changes in the concentration of extracellular calcium. K1 mRNA was expressed at low levels in cultures of keratinocytes growing on plastic in 0.05 mM calcium but in attached cells was not further induced by increases in the concentration of extracellular calcium. Suspension of the keratinocytes into semi-solid medium induced a rapid and substantial increase in both expression of K1 mRNA and in the percentage of cells expressing suprabasal keratin proteins. The induction was unaffected by the concentration of calcium in the semi-solid medium and could not be enhanced by exposing attached cells to higher calcium before suspension. The induction of K1 mRNA could be inhibited by exposure of the keratinocytes to either EGF or fibronectin. These results suggest that commitment of mouse keratinocytes to terminal differentiation is independent of extracellular calcium and may be regulated primarily by extracellular factors other than calcium.
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