ArticleCurrent microbiology1993
Amplification and sequencing of variable regions in bacterial 23S ribosomal RNA genes with conserved primer sequences.
Article in Current microbiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 62 citations in OpenAlex.
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- Neisseria arctica sp. nov., isolated from nonviable eggs of greater white-fronted geese (Anser albifrons) in Arctic Alaska.International journal of systematic and evolutionary microbiology · 2017Article
- Plesiomonas shigelloides Revisited.Clinical microbiology reviews · 2016Review
- leBIBIQBPP: a set of databases and a webtool for automatic phylogenetic analysis of prokaryotic sequences.BMC bioinformatics · 2015Article
- The rebirth of culture in microbiology through the example of culturomics to study human gut microbiota.Clinical microbiology reviews · 2015Article
- Evolutionary relationships among Chlamydophila abortus variant strains inferred by rRNA secondary structure-based phylogeny.PloS one · 2011Article
- The internal transcribed spacer region, a new tool for use in species differentiation and delineation of systematic relationships within the Campylobacter genus.Applied and environmental microbiology · 2010Article
- Molecular genetic basis of ribotyping.Clinical microbiology reviews · 2008Article
- Evaluation of 23S rRNA PCR primers for use in phylogenetic studies of bacterial diversity.Applied and environmental microbiology · 2006Article
- Neonatal infection-induced memory impairment after lipopolysaccharide in adulthood is prevented via caspase-1 inhibition.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Nodulation of Lupinus albus by strains of Ochrobactrum lupini sp. nov.Applied and environmental microbiology · 2005Article
- Two novel real-time reverse transcriptase PCR assays for rapid detection of bacterial contamination in platelet concentrates.Journal of clinical microbiology · 2004Article
- Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences.Journal of clinical microbiology · 2004Article
- Genetic diversity of Campylobacter jejuni isolates from farm animals and the farm environment.Applied and environmental microbiology · 2003Article
- Diagnostics of neisseriaceae and moraxellaceae by ribosomal DNA sequencing: ribosomal differentiation of medical microorganisms.Journal of clinical microbiology · 2001Article
- Specific PCR identification and differentiation of the thermophilic campylobacters, Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis.Journal of clinical microbiology · 1999Article
- Development of rRNA-targeted PCR and in situ hybridization with fluorescently labelled oligonucleotides for detection of Yersinia species.Journal of clinical microbiology · 1998Article
- Evaluation of a fluorescence-labelled oligonucleotide probe targeting 23S rRNA for in situ detection of Salmonella serovars in paraffin-embedded tissue sections and their rapid identification in bacterial smears.Journal of clinical microbiology · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Published bacterial 23S ribosomal RNA sequences were aligned, and universally conserved regions flanking highly variable regions were looked for. In strategically positioned conserved regions, six oligonucleotides suitable for polymerase chain reaction (PCR) and sequencing were designed, allowing fast sequencing of four of the most variable 23S rRNA regions. Two other primers were designed for PCR amplification of nearly complete 23S rRNA genes. All these primers successfully amplified fragments of 23S rRNA genes from seven unrelated bacteria. Four primers were used to determine 938 bp of sequence for Campylobacter jejuni subsp. jejuni. These results indicate that the oligonucleotide sequences presented here are useful for PCR amplification and sequence determination of variable 23S rRNA regions for a broad variety of eubacterial species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.