ArticleBiophysical journal1993
Restricted ion flow at the nuclear envelope of cardiac myocytes.
Article in Biophysical journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 29 citations in OpenAlex.
- Nuclear localization and functional characteristics of voltage-gated potassium channel Kv1.3.The Journal of biological chemistry · 2015Article
- Article
- Dendrimer-assisted patch-clamp sizing of nuclear pores.Pflugers Archiv : European journal of physiology · 2000Article
- Calcium, ATP and nuclear pore channel gating.Pflugers Archiv : European journal of physiology · 2000Article
- Nuclear hourglass technique: an approach that detects electrically open nuclear pores in Xenopus laevis oocyte.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Single-channel kinetics, inactivation, and spatial distribution of inositol trisphosphate (IP3) receptors in Xenopus oocyte nucleus.The Journal of general physiology · 1997Article
- Reconstitution of ionic channels from inner and outer membranes of mammalian cardiac nuclei.Biophysical journal · 1996Article
- Patch clamp and atomic force microscopy demonstrate TATA-binding protein (TBP) interactions with the nuclear pore complex.The Journal of membrane biology · 1995Article
- The ion channel behavior of the nuclear pore complex.The Journal of membrane biology · 1995Article
- Patch clamp detection of transcription factor translocation along the nuclear pore complex channel.The Journal of membrane biology · 1995Article
- Imaging nuclear pores of aldosterone-sensitive kidney cells by atomic force microscopy.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Nuclear electrophysiology.The Journal of membrane biology · 1994Review
- Open states of nuclear envelope ion channels in cardiac myocytes.The Journal of membrane biology · 1994Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flow of small ions across the nuclear envelope (NE) is thought to occur without restriction through large diameter nuclear pore complexes (NPCs). However, investigations with electron and fluorescence microscopy, and with patch-clamp and microelectrode electrophysiology, suggest that in many animal and plant cell types small ions move through a barrier having the signature of large conductance nuclear ion channels (NICs). As nucleocytoplasmic transport and gene activity are regulated by cytoplasmic signals and as it has recently been shown by this investigator that cardiac NICs are sensitive to cAMP-dependent processes (1), it was considered relevant to further investigate the effects of various cytosolic signals on NIC activity. Ion species substitution demonstrated that K+ is the major species responsible for NIC currents. The Na-channel blocker tetrodotoxin (TTX, 100 microM) and the Ca-channel blocker diltiazem (100 microM) had no effect, indicating no relation of NICs to Na- or Ca-channels in transit to the cell surface membrane. Zn2+ (100 microM) blocked NIC activity, suggesting a dual role in nucleocytoplasmic transport and gene function. GTP did not produce measurable effect. However, its nonhydrolyzable analogue GTP-gamma-S (10 microM) suppressed NIC activity, suggesting a role for GTP hydrolysis in NIC function. Deoxynucleotides (dNTPs, 200 microM) produced a transient increase in NIC activity, pointing to a modulation of NIC function by nucleic acid substrates. These results indicate a role for NICs in mediating: (a) control of gene activity by transduction and other cytosolic signals, and (b) nuclear demands and response to such signals.
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