ArticleThe Journal of cell biology1993
Polyamines are essential for cell transformation by pp60v-src: delineation of molecular events relevant for the transformed phenotype.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 73 citations in OpenAlex.
- A back-door insight into the modulation of Src kinase activity by the polyamine spermidine.eLife · 2023Article
- Air pollution exposure is associated with the gut microbiome as revealed by shotgun metagenomic sequencing.Environment international · 2020Article
- A Relay Pathway between Arginine and Tryptophan Metabolism Confers Immunosuppressive Properties on Dendritic Cells.Immunity · 2017Article
- Involvement of Antizyme Characterized from the Small Abalone Haliotis diversicolor in Gonadal Development.PloS one · 2015Article
- Amino acids regulate expression of antizyme-1 to modulate ornithine decarboxylase activity.The Journal of biological chemistry · 2012Article
- A prolonged and exaggerated wound response with elevated ODC activity mimics early tumor development.Carcinogenesis · 2011Article
- High expression of antizyme inhibitor 2, an activator of ornithine decarboxylase in steroidogenic cells of human gonads.Histochemistry and cell biology · 2009Article
- Antizyme and antizyme inhibitor, a regulatory tango.Cellular and molecular life sciences : CMLS · 2009Review
- EGFR plays a pivotal role in the regulation of polyamine-dependent apoptosis in intestinal epithelial cells.Cellular signalling · 2007Article
- Polyamines and nonmelanoma skin cancer.Toxicology and applied pharmacology · 2007Review
- Overexpression of antizyme-inhibitor in NIH3T3 fibroblasts provides growth advantage through neutralization of antizyme functions.Oncogene · 2006Article
- Regulation of all members of the antizyme family by antizyme inhibitor.The Biochemical journal · 2005Article
- High tumorigenic potential of a constitutively active mutant of the cholecystokinin 2 receptor.Oncogene · 2003Article
- Article
- A definitive role of ornithine decarboxylase in photocarcinogenesis.The American journal of pathology · 2001Article
- Manipulation of the expression of regulatory genes of polyamine metabolism results in specific alterations of the cell-cycle progression.The Biochemical journal · 2001Article
- Phosphorylated human keratinocyte ornithine decarboxylase is preferentially associated with insoluble cellular proteins.Molecular biology of the cell · 1999Article
- Anti-tumor activity of antizyme which targets the ornithine decarboxylase (ODC) required for cell growth and transformation.Oncogene · 1999Article
- [Nitric oxide, L-arginine and the kidney. Experimental studies of new therapy approaches].Medizinische Klinik (Munich, Germany : 1983) · 1998Review
- Specific requirement of putrescine for the mitogenic action of juvenile hormone on adult insect neuroblasts.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ornithine decarboxylase (ODC), the key enzyme of polyamine biosynthesis, becomes upregulated during cell proliferation and transformation. Here we show that intact ODC activity is needed for the acquisition of a transformed phenotype in rat 2R cells infected with a temperature-sensitive mutant of Rous sarcoma virus. Addition of the ODC inhibitor alpha-difluoromethyl ornithine (DFMO) to the cells (in polyamine-free medium) before shift to permissive temperature prevented the depolymerization of filamentous actin and morphological transformation. Polyamine supplementation restored the transforming potential of pp60v-src. DFMO did not interfere with the expression of pp60v-src or its in vitro tyrosine kinase activity. The tyrosine phosphorylation of most cellular proteins, including ras GAP, did not either display clear temperature- or DFMO-sensitive changes. A marked increase was, however, observed in the tyrosine phosphorylation of phosphatidylinositol 3-kinase and proteins of 33 and 36 kD upon the temperature shift, and these hyperphosphorylations were partially inhibited by DFMO. A DFMO-sensitive increase was also found in the total phosphorylation of calpactins I and II. The well-documented association of GAP with the phosphotyrosine-containing proteins p190 and p62 did not correlate with transformation, but a novel 42-kD tyrosine phosphorylated protein was complexed with GAP in a polyamine- and transformation-dependent manner. Further, tyrosine phosphorylated proteins of 130, 80/85, and 36 kD were found to coimmunoprecipitate with pp60v-src in a transformation-related manner. Altogether, this model offers a tool for sorting out the protein phosphorylations and associations critical for the transformed phenotype triggered by pp60v-src, and implicates a pivotal role for polyamines in cell transformation.
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