ArticleProceedings of the National Academy of Sciences of the United States of America1993
Epidermal growth factor and transforming growth factor alpha specifically induce the activation- and hyperproliferation-associated keratins 6 and 16.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
54 citing papers in PubMed, 1 synthesis or guideline pooled it, 189 citations in OpenAlex.
- Profiling and metaanalysis of epidermal keratinocytes responses to epidermal growth factor.BMC genomics · 2013Pooled it
- Scaffolds with spatiotemporally controlled growth factor delivery and cyclodextrin-enabled antagonism of growth factor receptor sequestration promote cutaneous wound healing.NPJ Regenerative medicine · 2025Article
- Keratins 6, 16, and 17 in Health and Disease: A Summary of Recent Findings.Current issues in molecular biology · 2024Review
- Growth factors and growth factor gene therapies for treating chronic wounds.Bioengineering & translational medicine · 2024Review
- Article
- Investigating iRHOM2-Associated Transcriptional Changes in Tylosis With Esophageal Cancer.Gastro hep advances · 2024Article
- Therapeutic role of growth factors in treating diabetic wound.World journal of diabetes · 2023Review
- Inhibiting EGFR Signaling Holds Promise for Treating Palmoplantar Keratodermas.The Journal of investigative dermatology · 2023Article
- Fluorapatite-Coated Percutaneous Devices Promote Wound Healing and Limit Epithelial Downgrowth at the Skin-Device Interface.Journal of tissue engineering and regenerative medicine · 2023Article
- Advances in molecular pathogenesis of hidradenitis suppurativa: Dysregulated keratins and ECM signaling.Seminars in cell & developmental biology · 2022Review
- Exosomes Derived From Umbilical Cord Mesenchymal Stem Cells Treat Cutaneous Nerve Damage and Promote Wound Healing.Frontiers in cellular neuroscience · 2022Article
- Review
- The Immunological Impact of IL-1 Family Cytokines on the Epidermal Barrier.Frontiers in immunology · 2021Review
- The Role of Multisystem Disease in Composition of Autologous Serum tears and ocular surface symptom improvement.The ocular surface · 2020Observational
- Mechanisms for Reducing Neuropathic Pain.Molecular neurobiology · 2020Review
- Topical application of omega-3-, omega-6-, and omega-9-rich oil emulsions for cutaneous wound healing in rats.Drug delivery and translational research · 2019Article
- Keratin 6a reorganization for ubiquitin-proteasomal processing is a direct antimicrobial response.The Journal of cell biology · 2018Article
- A hypomorphic Egfr allele does not ameliorate the palmoplantar keratoderma caused by SLURP1 deficiency.Experimental dermatology · 2017Article
- Hierarchy of cellular decisions in collective behavior: Implications for wound healing.Scientific reports · 2016Article
- Vimentin is necessary for colony growth of human diploid keratinocytes.Histochemistry and cell biology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Epidermal injury results in activation of keratinocytes which produce and respond to growth factors and cytokines and become migratory. Activated keratinocytes express a specific pair of keratin proteins, K6 and K16, distinct from the keratins in the healthy epidermis. Keratinocytes can be activated, for example, by binding of the appropriate ligands to the epidermal growth factor receptor (EGFR). We have analyzed the effects of EGFR activation on keratin gene transcription by transfecting DNAs containing keratin promoters linked to a reporter gene into primary cultures of human epidermal keratinocytes in the presence or absence of EGF or transforming growth factor alpha (TGF alpha), two growth factors that activate EGFR. The activation of EGFR had no effect on the promoters of simple epithelial, basal-layer-specific, or differentiation-specific keratins. In contrast, the expression of K6 and K16 was strongly and specifically induced. A 20-bp DNA segment of the K16 gene promoter conveyed the EGF regulation, functioned in a heterologous construct, and therefore constituted an EGF-responsive element. A nuclear protein specifically bound to this element and to the analogous sequence of the K6 promoter. Thus, EGF specifically induces K6 and K16, markers of activated keratinocytes, via nuclear proteins that bind to EGF-responsive elements in the promoters of these keratin genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.