ArticleProceedings of the National Academy of Sciences of the United States of America1993
Expression and localization of two low molecular weight GTP-binding proteins, Rab8 and Rab10, by epitope tag.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 148 citations in OpenAlex.
- The role of abnormal epigenetic regulation of small GTPases in glioma (Review).International journal of oncology · 2025Review
- Receptor-mediated internalization promotes increased endosome size and number in a RAB4- and RAB5-dependent manner.European journal of cell biology · 2023Article
- Rab GTPases: The principal players in crafting the regulatory landscape of endosomal trafficking.Computational and structural biotechnology journal · 2022Review
- Rab35 Targeting to the Plasma Membrane Is Dependent on the C-terminal Polybasic Cluster.Acta histochemica et cytochemica · 2020Article
- In vivo mapping of a GPCR interactome using knockin mice.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- TBC1D21 Potentially Interacts with and Regulates Rap1 during Murine Spermatogenesis.International journal of molecular sciences · 2018Article
- Retracted Article: Down-regulation of Rab10 inhibits hypoxia-induced invasion and EMT in thyroid cancer cells by targeting HIF-1α through the PI3K/Akt pathway.RSC advances · 2018Article
- Rab 10-a traffic controller in multiple cellular pathways and locations.Journal of cellular physiology · 2018Review
- RAB10 overexpression promotes tumor growth and indicates poor prognosis of hepatocellular carcinoma.Oncotarget · 2017Article
- RAB10 Interacts with the Male Germ Cell-Specific GTPase-Activating Protein during Mammalian Spermiogenesis.International journal of molecular sciences · 2017Article
- Defining the molecular basis of interaction between R3 receptor-type protein tyrosine phosphatases and VE-cadherin.PloS one · 2017Article
- Endocytosis and Enamel Formation.Frontiers in physiology · 2017Review
- Endoplasmic reticulum-resident Rab8A GTPase is involved in phagocytosis in the protozoan parasite Entamoeba histolytica.Cellular microbiology · 2016Article
- Article
- DhhP, a cyclic di-AMP phosphodiesterase of Borrelia burgdorferi, is essential for cell growth and virulence.Infection and immunity · 2014Article
- Expression and detection of LINE-1 ORF-encoded proteins.Mobile genetic elements · 2014Article
- Article
- Expression of long-form N-acetylglucosamine-6-O-sulfotransferase 1 in human high endothelial venules.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Article
- The first human epitope map of the alphaviral E1 and E2 proteins reveals a new E2 epitope with significant virus neutralizing activity.PLoS neglected tropical diseases · 2010Article
- Calreticulin-dependent recycling in the early secretory pathway mediates optimal peptide loading of MHC class I molecules.The EMBO journal · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Small GTP-binding proteins of the YPT/SEC4/Rab family have been shown to play an essential role in intracellular membrane trafficking. In mammals, Rab8 and Rab10 are the two small GTP-binding proteins identified so far that are closest to SEC4, an essential gene product involved in post-Golgi constitutive secretion in the yeast Saccharomyces cerevisiae. To study the localization of Rab proteins, we have expressed the cDNAs with an influenza virus hemagglutinin (HA) epitope tag at the N terminus. The feasibility of this method was tested by using yeast SEC4. HA-tagged SEC4 functionally complemented a temperature-sensitive sec4 mutant similarly to wild-type SEC4, indicating that the modified protein retained functional integrity. Monoclonal antibody 12CA5, raised against the HA tag, was used to determine the expression and localization of HA-tagged proteins after transfection. In stably transfected CHO and Swiss 3T3 cells, HA-tagged Rab8 was localized to the cell periphery, with the highest concentration in the ruffling areas. In contrast, epitope-tagged Rab10 expressed in CHO and BHK cells was concentrated on membranes in the perinuclear region. By light microscopy, the staining partially overlapped with that of a Golgi marker, beta-COP. Thus, despite the high degree homology of Rab8 and Rab10 (66% identity), the two proteins are localized to distinct cellular compartments. This approach should provide a general tool for the analyses of other members of the YPT/SEC4/rab gene family.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.