ArticleJournal of bacteriology1993
Gene inactivation in Lactococcus lactis: histidine biosynthesis.
Article in Journal of bacteriology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- Computational Identification of Novel Transcriptional Regulators and Functional Gene Clusters inMicroorganisms · 2026Article
- Global diversity of enterococci and description of 18 previously unknown species.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Microbial interactions shape cheese flavour formation.Nature communications · 2023Article
- Recombination-mediated remodelling of host-pathogen interactions duringMicrobial genomics · 2015Article
- Physiology and substrate specificity of two closely related amino acid transporters, SerP1 and SerP2, of Lactococcus lactis.Journal of bacteriology · 2015Article
- Cloning, expression, and functional characterization of secondary amino acid transporters of Lactococcus lactis.Journal of bacteriology · 2013Article
- Evolutionary blueprint for host- and niche-adaptation in Staphylococcus aureus clonal complex CC30.Frontiers in cellular and infection microbiology · 2012Article
- Pilus biogenesis in Lactococcus lactis: molecular characterization and role in aggregation and biofilm formation.PloS one · 2012Article
- Microbial domestication signatures of Lactococcus lactis can be reproduced by experimental evolution.Genome research · 2012Article
- Investigation of the adaptation of Lactococcus lactis to isoleucine starvation integrating dynamic transcriptome and proteome information.Microbial cell factories · 2011Article
- Systems biology of lactic acid bacteria: a critical review.Microbial cell factories · 2011Review
- Genome-scale diversity and niche adaptation analysis of Lactococcus lactis by comparative genome hybridization using multi-strain arrays.Microbial biotechnology · 2011Article
- Regulatory phenotyping reveals important diversity within the species Lactococcus lactis.Applied and environmental microbiology · 2009Article
- Complementation of the Lactococcus lactis secretion machinery with Bacillus subtilis SecDF improves secretion of staphylococcal nuclease.Applied and environmental microbiology · 2006Article
- Sulfur amino acid metabolism and its control in Lactococcus lactis IL1403.Journal of bacteriology · 2005Article
- Psi-Phi: exploring the outer limits of bacterial pseudogenes.Genome research · 2004Article
- Influence of lipoteichoic acid D-alanylation on protein secretion in Lactococcus lactis as revealed by random mutagenesis.Applied and environmental microbiology · 2004Article
- Extent of genetic lesions of the arginine and pyrimidine biosynthetic pathways in Lactobacillus plantarum, L. paraplantarum, L. pentosus, and L. casei: prevalence of CO(2)-dependent auxotrophs and characterization of deficient arg genes in L. plantarum.Applied and environmental microbiology · 2003Article
- Molecular and functional analyses of the metC gene of Lactococcus lactis, encoding cystathionine beta-lyase.Applied and environmental microbiology · 2000Article
- Regulation of expression of the Lactococcus lactis histidine operon.Journal of bacteriology · 1999Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lactococcus lactis strains from dairy and nondairy sources were tested for the ability to grow in the absence of histidine. Among 60 dairy strains tested, 56 required histidine, whereas only 1 of 11 nondairy strains had this requirement. Moreover, 10 of the 56 auxotrophic strains were able to grow in the presence of histidinol (Hol+), the immediate histidine precursor. This indicates that adaptation to milk often results in histidine auxotrophy. The histidine operon was detected by Southern hybridization in eight dairy auxotrophic strains tested. A large part of the histidine operon (8 kb, containing seven histidine biosynthetic genes and three unrelated open reading frames [ORFs]) was cloned from an auxotroph, which had an inactive hisD gene, as judged by its inability to grow on histidinol. Complementation analysis of three genes, hisA, hisB, and hisG, in Escherichia coli showed that they also were inactive. Sequence analysis of the cloned histidine region, which revealed 98.6% overall homology with that of the previously analyzed prototrophic strain, showed the presence of frameshift mutations in three his genes, hisC, hisG, and hisH, and two genes unrelated to histidine biosynthesis, ORF3 and ORF6. In addition, several mutations were detected in the promoter region of the operon. Northern (RNA) hybridization analysis showed a much lower amount of the his transcript in the auxotrophic strain than in the prototrophic strain. The mutations detected account for the histidine auxotrophy of the analyzed strain. Certain other dairy auxotrophic strains carry a lower number of mutations, since they were able to revert either to a Hol+ phenotype or to histidine prototrophy.
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