ArticleThe Journal of cell biology1993
Dynamics of keratin assembly: exogenous type I keratin rapidly associates with type II keratin in vivo.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 59 citations in OpenAlex.
- Review
- Emerging roles of sumoylation in the regulation of actin, microtubules, intermediate filaments, and septins.Cytoskeleton (Hoboken, N.J.) · 2015Review
- Kinetic Monte Carlo simulations of the assembly of filamentous biomacromolecules by dimer addition mechanism.RSC advances · 2015Article
- Measuring the regulation of keratin filament network dynamics.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Inroads into the structure and function of intermediate filament networks.Journal of structural biology · 2012Review
- Insights into the mechanical properties of epithelial cells: the effects of shear stress on the assembly and remodeling of keratin intermediate filaments.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2009Article
- Intermediate filaments: versatile building blocks of cell structure.Current opinion in cell biology · 2008Review
- Ichthyosis update: towards a function-driven model of pathogenesis of the disorders of cornification and the role of corneocyte proteins in these disorders.Advances in dermatology · 2007Review
- Identification of novel principles of keratin filament network turnover in living cells.Molecular biology of the cell · 2004Article
- Insights into the dynamic properties of keratin intermediate filaments in living epithelial cells.The Journal of cell biology · 2001Article
- Motile properties of vimentin intermediate filament networks in living cells.The Journal of cell biology · 1998Article
- Disruption of nuclear lamin organization alters the distribution of replication factors and inhibits DNA synthesis.The Journal of cell biology · 1997Article
- Intermediate filaments as dynamic structures.Cancer metastasis reviews · 1996Review
- The function of intermediate filaments in cell shape and cytoskeletal integrity.The Journal of cell biology · 1996Article
- A nontetrameric species is the major soluble form of keratin in Xenopus oocytes and rabbit reticulocyte lysates.The Journal of cell biology · 1996Article
- Determinants for intracellular sorting of cytoplasmic and nuclear intermediate filaments.The Journal of cell biology · 1994Article
- Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein.The Journal of cell biology · 1994Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Keratin intermediate filaments (IF) are obligate heteropolymers containing equal amounts of type I and type II keratin. We have previously shown that microinjected biotinylated type I keratin is rapidly incorporated into endogenous bundles of keratin IF (tonofilaments) of PtK2 cells. In this study we show that the earliest steps in the assembly of keratin subunits into tonofilaments involve the extremely rapid formation of discrete aggregates of microinjected keratin. These are seen as fluorescent spots containing both type I and type II keratins within 1 min post-injection as determined by double label immunofluorescence. These observations suggest that endogenous type II keratin subunits can be rapidly mobilized from their endogenous state to form complexes with the injected type I protein. Furthermore, confocal microscopy and immunogold electron microscopy suggest that the type I-type II keratin spots from in close association with the endogenous keratin IF network. When the biotinylated protein is injected at concentrations of 0.3-0.5 mg/ml, the organization of the endogenous network of tonofilaments remains undisturbed during incorporation into tonofilaments. However, microinjection of 1.5-2.0 mg/ml of biotinylated type I results in significant alterations in the organization and assembly state of the endogenous keratin IF network soon after microinjection. The results of this study are consistent with the existence of a state of equilibrium between keratin subunits and polymerized keratin IF in epithelial cells, and provide further proof that IF are dynamic elements of the cytoskeleton of mammalian cells.
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