ArticleThe Journal of membrane biology1993
Cytoskeleton and ion movements during volume regulation in cultured PC12 cells.
Article in The Journal of membrane biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 64 citations in OpenAlex.
- Identification of key signaling molecules involved in the activation of the swelling-activated chloride current in human glioblastoma cells.The Journal of membrane biology · 2014Article
- Hypotonic activation of volume-sensitive outwardly rectifying anion channels (VSOACs) requires coordinated remodeling of subcortical and perinuclear actin filaments.The Journal of membrane biology · 2005Article
- Regulation of the cellular content of the organic osmolyte taurine in mammalian cells.Neurochemical research · 2004Review
- Reduced intracellular ionic strength as the initial trigger for activation of endothelial volume-regulated anion channels.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Brain accumulation of myo-inositol in the trisomy 16 mouse, an animal model of Down's syndrome.The Biochemical journal · 1996Article
- Swelling-induced catecholamine secretion recorded from single chromaffin cells.Pflugers Archiv : European journal of physiology · 1995Article
- Modulation of a volume-regulated chloride current by F-actin.The Journal of membrane biology · 1995Article
- Human macrophages contain a stretch-sensitive potassium channel that is activated by adherence and cytokines.The Journal of membrane biology · 1995Article
- Molecular basis of epithelial Cl channels.The Journal of membrane biology · 1995Review
- Ca2+ channel Ca(2+)-dependent inactivation in a mammalian central neuron involves the cytoskeleton.Pflugers Archiv : European journal of physiology · 1994Article
- Evidence for a regulatory protein involved in the increased activity of system A for neutral amino acid transport in osmotically stressed mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Morphological alterations and cytoskeletal reorganization in opossum kidney (OK) cells during osmotic swelling and volume regulation.Histochemistry · 1994Article
- Regulatory and molecular aspects of mammalian amino acid transport.The Biochemical journal · 1994Review
- Relation between cytoskeleton, hypo-osmotic treatment and volume regulation in Ehrlich ascites tumor cells.The Journal of membrane biology · 1993Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The present study investigates the role of cytoskeletal elements, microtubules and microfilaments, on ion transport systems activated during volume regulatory processes in PC12 pheochromocytoma cells. Disruption of microtubule network by colchicine (0.1 mM) or vinblastine sulfate (10 microM) has no significant effect on PC12 cell hydration or on changes of the intracellular K+, Cl- and Na+ content observed in hypo-osmotic conditions. Disruption of microfilament network by cytochalasin B strongly affects volume regulation in a dose-dependent manner. Cytochalasin B leads to a potentiation of the initial cell swelling and the regulatory volume decrease is suppressed. Although, the internal K+ and Cl- level decreases significantly, as demonstrated by measurements of intracellular ion content and 86Rb fluxes. Using the patch-clamp technique, we could demonstrate in PC12 cell membranes an ion channel whose gating is affected by application of a negative hydrostatic pressure (mechanical stress) to the membrane patch, by exposure of the cell to hypoosmotic medium (osmotic stress), or by disruption of the microfilament network with cytochalasin B.
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