ArticleThe Journal of cell biology1993
Glycosylphosphatidylinositol-anchored proteins are preferentially targeted to the basolateral surface in Fischer rat thyroid epithelial cells.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 167 citations in OpenAlex.
- ACE-tRNAs are a platform technology for suppressing nonsense mutations that cause cystic fibrosis.Nucleic acids research · 2025Article
- Emerging connections between GPI-anchored proteins and their extracellular carriers in colorectal cancer.Extracellular vesicles and circulating nucleic acids · 2023Article
- Self-organization of apical membrane protein sorting in epithelial cells.The FEBS journal · 2022Article
- A posttranslational modification code for CFTR maturation is altered in cystic fibrosis.Science signaling · 2019Article
- Trafficking of glycosylphosphatidylinositol anchored proteins from the endoplasmic reticulum to the cell surface.Journal of lipid research · 2016Review
- GPI-anchored protein organization and dynamics at the cell surface.Journal of lipid research · 2016Review
- Chiara Zurzolo: GPI knows the way to go.The Journal of cell biology · 2015Article
- Hepatocyte polarity.Comprehensive Physiology · 2013Review
- Polarized sorting and trafficking in epithelial cells.Cell research · 2012Review
- N-Glycosylation instead of cholesterol mediates oligomerization and apical sorting of GPI-APs in FRT cells.Molecular biology of the cell · 2011Article
- Apical trafficking in epithelial cells: signals, clusters and motors.Journal of cell science · 2009Review
- Lipid rafts and clathrin cooperate in the internalization of PrP in epithelial FRT cells.PloS one · 2009Article
- Selective roles for cholesterol and actin in compartmentalization of different proteins in the Golgi and plasma membrane of polarized cells.The Journal of biological chemistry · 2008Article
- The glycosylphosphatidylinositol anchor: a complex membrane-anchoring structure for proteins.Biochemistry · 2008Review
- Antibody to AP1B adaptor blocks biosynthetic and recycling routes of basolateral proteins at recycling endosomes.Molecular biology of the cell · 2007Article
- Controversy fuels trafficking of GPI-anchored proteins.The Journal of cell biology · 2006Article
- Functional interaction between p75NTR and TrkA: the endocytic trafficking of p75NTR is driven by TrkA and regulates TrkA-mediated signalling.The Biochemical journal · 2005Article
- Protein oligomerization modulates raft partitioning and apical sorting of GPI-anchored proteins.The Journal of cell biology · 2004Article
- PrP(C) association with lipid rafts in the early secretory pathway stabilizes its cellular conformation.Molecular biology of the cell · 2004Article
- Prion infection of epithelial Rov cells is a polarized event.Journal of virology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Glycosylphosphatidylinositol (GPI) acts as an apical targeting signal in MDCK cells and other kidney and intestinal cell lines. In striking contrast with these model polarized cell lines, we show here that Fischer rat thyroid (FRT) epithelial cells do not display a preferential apical distribution of GPI-anchored proteins. Six out of nine detectable endogenous GPI-anchored proteins were localized on the basolateral surface, whereas two others were apical and one was not polarized. Transfection of several model GPI proteins, previously shown to be apically targeted in MDCK cells, also led to unexpected results. While the ectodomain of decay accelerating factor (DAF) was apically secreted, 50% of the native, GPI-anchored form, of this protein was basolateral. Addition of a GPI anchor to the ectodomain of Herpes simplex gD-1, secreted without polarity, led to basolateral localization of the fusion protein, gD1-DAF. Targeting experiments demonstrated that gD1-DAF was delivered vectorially from the Golgi apparatus to the basolateral surface. These results indicate that FRT cells have fundamental differences with MDCK cells with regard to the mechanisms for sorting GPI-anchored proteins: GPI is not an apical signal but, rather, it behaves as a basolateral signal. The "mutant" behavior of FRT cells may provide clues to the nature of the mechanisms that sort GPI-anchored proteins in epithelial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.