ArticleMolecular and cellular biology1993
Probing keratinocyte and differentiation specificity of the human K5 promoter in vitro and in transgenic mice.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 133 citations in OpenAlex.
- Trial
- Studying Hair Growth in Mice: Synchronization of Hair Follicle Growth by Depilation.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Keratins as an Inflammation Trigger Point in Epidermolysis Bullosa Simplex.International journal of molecular sciences · 2021Review
- Two-photon microscopy for intracutaneous imaging of stem cell activity in mice.Experimental dermatology · 2017Review
- Dermal Delivery of Constructs Encoding Cre Recombinase to Induce Skin Tumors in PtenInternational journal of molecular sciences · 2016Article
- Analysis of compound heterozygotes reveals that the mouse floxed Pax6 (tm1Ued) allele produces abnormal eye phenotypes.Transgenic research · 2016Article
- Characterization of the Promoter Regions of Two Sheep Keratin-Associated Protein Genes for Hair Cortex-Specific Expression.PloS one · 2016Article
- Atypical Protein Kinase C Isoform, aPKCλ, Is Essential for Maintaining Hair Follicle Stem Cell Quiescence.The Journal of investigative dermatology · 2015Article
- Cell surface marker profiling of human tracheal basal cells reveals distinct subpopulations, identifies MST1/MSP as a mitogenic signal, and identifies new biomarkers for lung squamous cell carcinomas.Respiratory research · 2014Article
- Modeling cutaneous squamous carcinoma development in the mouse.Cold Spring Harbor perspectives in medicine · 2014Review
- Progesterone-inducible cytokeratin 5-positive cells in luminal breast cancer exhibit progenitor properties.Hormones & cancer · 2013Article
- Role of stat3 in skin carcinogenesis: insights gained from relevant mouse models.Journal of skin cancer · 2013Article
- Smad4 disruption accelerates keratinocyte reepithelialization in murine cutaneous wound repair.Histochemistry and cell biology · 2012Article
- A high-content assay to identify small-molecule modulators of a cancer stem cell population in luminal breast cancer.Journal of biomolecular screening · 2012Article
- The a"MAZE"ing world of lung-specific transgenic mice.American journal of respiratory cell and molecular biology · 2012Review
- Stem cells and corneal epithelial maintenance: insights from the mouse and other animal models.Results and problems in cell differentiation · 2012Review
- Interferon regulatory factor 6 is necessary, but not sufficient, for keratinocyte differentiation.The Journal of investigative dermatology · 2012Article
- Varying phenotypes in swine versus murine transgenic models constitutively expressing the same human Sonic hedgehog transcriptional activator, K5-HGLI2 Delta N.Transgenic research · 2010Article
- Genetic analysis of cytoprotective functions supported by graded expression of Keap1.Molecular and cellular biology · 2010Article
- Melanocyte-like cells in the heart and pulmonary veins contribute to atrial arrhythmia triggers.The Journal of clinical investigation · 2009Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Keratins K5 and K14 form the extensive intermediate filament network of mitotically active basal cells in all stratified epithelia. We have explored the regulatory mechanisms governing cell-type-specific and differentiation stage-specific expression of the human K5 gene in transiently transfected keratinocytes in vitro and in transgenic mice in vivo. Six thousand base pairs of 5' upstream K5 sequence directed proper basal cell-specific expression in all stratified epithelia. Surprisingly, as few as 90 bp of the K5 promoter still directed expression to stratified epithelia, with expression predominantly in epidermis, hair follicles, and tongue. Despite keratinocyte-preferred expression, the truncated K5 promoter displayed departures from basal to suprabasal expression in epidermis and from outer root sheath to inner root sheath expression in the follicle, with some regional variations in expression as well. To begin to elucidate the molecular controls underlying the keratinocyte specificity of the truncated promoter, we examined protein-DNA interactions within this region. A number of keratinocyte nuclear proteins bind to a K5 gene segment extending from -90 to +32 bp and are functionally involved in transcriptional regulation in vitro. Interestingly, several of these factors are common to both the K5 and K14 promoters, although they appear to be distinct from those previously implicated in keratinocyte specificity. Mutagenesis studies indicate that factors binding in the vicinity of the TATA box and transcription initiation are responsible for the cell type specificity of the truncated K5 promoter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.