ArticleThe American journal of pathology1993
Proliferating cell nuclear antigen immunohistochemistry using monoclonal antibody 19A2 and a new antigen retrieval technique has prognostic impact in archival paraffin-embedded node-negative breast cancer.
Article in The American journal of pathology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 76 citations in OpenAlex.
- Immunohistochemical expression of dog TERT in canine testicular tumours in relation to PCNA, ki67 and p53 expression.Veterinary research communications · 2009Article
- Interaction of estrogen receptor alpha with proliferating cell nuclear antigen.Nucleic acids research · 2007Article
- Impact of simultaneous assay, the PCNA, cyclinD1, and DNA content with specimens before and after preoperative radiotherapy on prognosis of esophageal cancer-possible incorporation into clinical TNM staging system.World journal of gastroenterology · 2005Article
- Tumor growth inhibition effect of hIL-6 on colon cancer cells transfected with the target gene by retroviral vector.World journal of gastroenterology · 2000Article
- Clinicopathologic study associated with long-term survival in Japanese patients with node-negative breast cancer.British journal of cancer · 2000Article
- Proliferation markers in tumours: interpretation and clinical value.Journal of clinical pathology · 1998Review
- Immunohistochemical study on the prognostic value of MIB-1 in gastric carcinoma.British journal of cancer · 1996Article
- Up-regulated expression of transforming growth factor-alpha in the bronchiolar-alveolar duct regions of asbestos-exposed rats.The American journal of pathology · 1996Article
- Proliferative activity of mixed hyperplastic adenomatous polyp/serrated adenoma in the large intestine, measured by PCNA (proliferating cell nuclear antigen).Journal of gastroenterology · 1996Article
- Correlation of growth fraction by Ki-67 and proliferating cell nuclear antigen (PCNA) immunohistochemistry with histopathological parameters and prognosis in primary breast carcinomas.Breast cancer research and treatment · 1996Article
- Outcome of 31 intracranial haemangiopericytomas: poor predictive value of cell proliferation indices.Acta neurochirurgica · 1996Article
- Quantitative histopathology in lymph node-negative breast cancer. Prognostic significance of mitotic counts.Virchows Archiv : an international journal of pathology · 1995Article
- Axonal regeneration into chronically denervated distal stump. 2. Active expression of type I collagen mRNA in epineurium.Acta neuropathologica · 1995Article
- Prognostic value of proliferating cell nuclear antigen and c-erbB-2 compared with conventional histopathological factors in breast cancer.Journal of cancer research and clinical oncology · 1995Article
- Proliferative activity is a significant prognostic factor in male breast carcinoma.The American journal of pathology · 1994Article
- Proliferating cell nuclear antigen in breast carcinomas. An immunohistochemical study with correlation to histopathological features and prognostic factors.Virchows Archiv : an international journal of pathology · 1994Article
- Update on proliferation-associated antibodies applicable to formalin-fixed paraffin-embedded tissue and their clinical applications.The Histochemical journal · 1993Review
- Aberrant p53 expression in astrocytic neoplasms of the brain: association with proliferation.The American journal of pathology · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We evaluated whether proliferating cell nuclear antigen (PCNA) immunohistochemistry with antigen retrieval could be used as a measure of cell proliferation in archival, formalin-fixed, paraffin-embedded tissues and whether the staining results have long-term prognostic significance in axillary node-negative breast cancer. Primary tumor samples obtained from 109 axillary-node-negative breast cancer cases were used for the study. The best staining results were obtained with the 19A2 antibody after microwave heating in a solution of saturated lead thiocyanate. Using this method, there was a significant correlation (linear regression, r = 0.580, P < 0.001) between the proportion of PCNA19A2-positive carcinoma cells (PCNA19A2 score) and DNA flow cytometric S phase fraction. A high PCNA19A2 score was associated with high mitotic count, DNA aneuploidy, and absence of estrogen receptors. Axillary-node-negative patients with a high PCNA19A2 score (cut-point 8%) had significantly worse prognosis than those with a low PCNA19A2 score (P = 0.008). According to a Cox multivariate analysis, PCNA19A2 score had independent prognostic value but only if S phase fraction was excluded from the analysis. In our study, the PCNAPC10 score correlated weakly only with primary tumor size (analysis of variance) and prognosis (5-year univariate survival analysis), but the significance of these findings needs further evaluation. In conclusion, PCNA immunohistochemistry with the 19A2 antibody after an appropriate antigen retrieval treatment may offer a useful alternative to DNA flow cytometry for the analysis of cell proliferation activity from formalin-fixed, paraffin-embedded breast carcinomas.
Indexed as
Identifiers
7682759PMC1886856W1600992032What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.