ArticleProceedings of the National Academy of Sciences of the United States of America1993
Thy-1 glycoprotein and src-like protein-tyrosine kinase p53/p56lyn are associated in large detergent-resistant complexes in rat basophilic leukemia cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 124 citations in OpenAlex.
- Conformational coupling of integrin and Thy-1 regulates Fyn priming and fibroblast mechanotransduction.The Journal of cell biology · 2015Article
- PAG--a multipurpose transmembrane adaptor protein.Oncogene · 2014Review
- Article
- Spatio-temporal signaling in mast cells.Advances in experimental medicine and biology · 2011Review
- Combined chemical and enzymatic stable isotope labeling for quantitative profiling of detergent-insoluble membrane proteins isolated using Triton X-100 and Brij-96.Journal of proteome research · 2006Article
- Markers for detergent-resistant lipid rafts occupy distinct and dynamic domains in native membranes.Molecular biology of the cell · 2004Article
- Isolation and characterization of lipid rafts with different properties from RBL-2H3 (rat basophilic leukaemia) cells.The Biochemical journal · 2004Article
- Positive and negative regulation of T-cell activation through kinases and phosphatases.The Biochemical journal · 2003Review
- Relationship of lipid rafts to transient confinement zones detected by single particle tracking.Biophysical journal · 2002Article
- Glypican-2 binds to midkine: the role of glypican-2 in neuronal cell adhesion and neurite outgrowth.Glycoconjugate journal · 2001Article
- Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase csk and is involved in regulation of T cell activation.The Journal of experimental medicine · 2000Article
- Critical role for cholesterol in Lyn-mediated tyrosine phosphorylation of FcepsilonRI and their association with detergent-resistant membranes.The Journal of cell biology · 1999Article
- Microdomain-dependent regulation of Lck and Fyn protein-tyrosine kinases in T lymphocyte plasma membranes.Molecular biology of the cell · 1999Article
- The triggering signal dictates the effect of docosahexaenoic acid on lymphocyte function in vitro.Lipids · 1998Article
- Exclusion of CD45 inhibits activity of p56lck associated with glycolipid-enriched membrane domains.The Journal of cell biology · 1996Article
- Intracellular signaling is changed after clustering of the neural cell adhesion molecules axonin-1 and NgCAM during neurite fasciculation.The Journal of cell biology · 1996Article
- Fc epsilon RI-mediated association of 6-micron beads with RBL-2H3 mast cells results in exclusion of signaling proteins from the forming phagosome and abrogation of normal downstream signaling.The Journal of cell biology · 1996Article
- Thy-1-mediated activation of rat mast cells: the role of Thy-1 membrane microdomains.Immunology · 1996Article
- Endocytosis of GPI-linked membrane folate receptor-alpha.The Journal of cell biology · 1996Article
- Fc epsilon RI-mediated recruitment of p53/56lyn to detergent-resistant membrane domains accompanies cellular signaling.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thy-1 is a surface glycoprotein that is attached to the plasma membrane by a glycosyl-phosphatidyl-inositol anchor. Crosslinking of Thy-1 in rat mast cells and basophilic leukemia cells (RBL-2H3) induces cell activation including histamine release and tyrosine phosphorylation of several proteins. Here we show that glycosyl-phosphatidylinositol-linked Thy-1 forms noncovalent complexes with src-related protein-tyrosine kinase p53/p56lyn and other protein-tyrosine kinases and/or their substrates. These complexes are resistant to solubilization by a nonionic detergent, sedimentable at 200,000 x g, and very large ( > 10 MDa) as determined by gel chromatography. Activation of RBL-2H3 cells by crosslinking of the high-affinity IgE receptors resulted in decreased recovery of the complexes. The combined data indicate the existence of large detergent-resistant domains in the surface membrane of mast cells that may play an important role in their activation.
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