ArticleMolecular and cellular biology1993
In vivo recognition of a vertebrate mini-exon as an exon-intron-exon unit.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
35 citing papers in PubMed, 81 citations in OpenAlex.
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- Transcription initiation of distant core promoters in a large-sized genome of an insect.BMC biology · 2021Article
- In silico analysis of the sequence features responsible for alternatively spliced introns in the model green alga Chlamydomonas reinhardtii.Plant molecular biology · 2017Article
- Investigation of SCGB3A1 (UGRP2) gene arrays in patients with nasal polyposis.European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery · 2014Article
- Experimental assessment of splicing variants using expression minigenes and comparison with in silico predictions.Human mutation · 2014Article
- HnRNPH1/H2, U1 snRNP, and U11 snRNP cooperate to regulate the stability of the U11-48K pre-mRNA.RNA (New York, N.Y.) · 2013Article
- Sequence features responsible for intron retention in human.BMC genomics · 2007Article
- Synonymous SNPs provide evidence for selective constraint on human exonic splicing enhancers.Journal of molecular evolution · 2006Article
- The architecture of pre-mRNAs affects mechanisms of splice-site pairing.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Sequence conservation, relative isoform frequencies, and nonsense-mediated decay in evolutionarily conserved alternative splicing.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Computational discovery of internal micro-exons.Genome research · 2003Article
- SR proteins Asf/SF2 and 9G8 interact to activate enhancer-dependent intron D splicing of bovine growth hormone pre-mRNA in vitro.RNA (New York, N.Y.) · 2000Article
- Evidence for splice site pairing via intron definition in Schizosaccharomyces pombe.Molecular and cellular biology · 2000Article
- Multiple splicing defects in an intronic false exon.Molecular and cellular biology · 2000Article
- Human genomic sequences that inhibit splicing.Molecular and cellular biology · 2000Article
- A 5' splice site-proximal enhancer binds SF1 and activates exon bridging of a microexon.Molecular and cellular biology · 2000Article
- Cold shock induces the insertion of a cryptic exon in the neurofibromatosis type 1 (NF1) mRNA.Nucleic acids research · 2000Article
- Requirements for mini-exon inclusion in potato invertase mRNAs provides evidence for exon-scanning interactions in plants.RNA (New York, N.Y.) · 2000Article
- Sensitivity of splice sites to antisense oligonucleotides in vivo.RNA (New York, N.Y.) · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Very small vertebrate exons are problematic for RNA splicing because of the proximity of their 3' and 5' splice sites. In this study, we investigated the recognition of a constitutive 7-nucleotide mini-exon from the troponin I gene that resides quite close to the adjacent upstream exon. The mini-exon failed to be included in spliced RNA when placed in a heterologous gene unless accompanied by the upstream exon. The requirement for the upstream exon disappeared when the mini-exon was internally expanded, suggesting that the splice sites bordering the mini-exon are compatible with those of other constitutive vertebrate exons and that the small size of the exon impaired inclusion. Mutation of the 5' splice site of the natural upstream exon did not result in either exon skipping or activation of a cryptic 5' splice site, the normal vertebrate phenotypes for such mutants. Instead, a spliced RNA accumulated that still contained the upstream intron. In vitro, the mini-exon failed to assemble into spliceosome complexes unless either internally expanded or accompanied by the upstream exon. Thus, impaired usage of the mini-exon in vivo was accompanied by impaired recognition in vitro, and recognition of the mini-exon was facilitated by the presence of the upstream exon in vivo and in vitro. Cumulatively, the atypical in vivo and in vitro properties of the troponin exons suggest a mechanism for the recognition of this mini-exon in which initial recognition of an exon-intron-exon unit is followed by subsequent recognition of the intron.
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