ArticleChromosoma1993
Three-dimensional organization of the ribosomal genes and Ag-NOR proteins during interphase and mitosis in PtK1 cells studied by confocal microscopy.
Article in Chromosoma, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 56 citations in OpenAlex.
- Fragile Sites of 'Valencia' Sweet Orange (Citrus sinensis) Chromosomes Are Related with Active 45s rDNA.PloS one · 2016Article
- Chromosome breakages associated with 45S ribosomal DNA sequences in spotted snakehead fish Channa punctatus.Molecular biology reports · 2013Article
- Plant 45S rDNA clusters are fragile sites and their instability is associated with epigenetic alterations.PloS one · 2012Article
- Article
- Aurora-B regulates RNA methyltransferase NSUN2.Molecular biology of the cell · 2007Article
- Transcription factor YY1 associates with pericentromeric gamma-satellite DNA in cycling but not in quiescent (G0) cells.Nucleic acids research · 2004Article
- The three-dimensional study of chromosomes and upstream binding factor-immunolabeled nucleolar organizer regions demonstrates their nonrandom spatial arrangement during mitosis.Molecular biology of the cell · 1998Article
- Electron tomography of metaphase nucleolar organizer regions: evidence for a twisted-loop organization.Molecular biology of the cell · 1997Article
- Relative distribution of rDNA and proteins of the RNA polymerase I transcription machinery at chromosomal NORs.Chromosoma · 1997Article
- Involvement of in situ conformation of ribosomal genes and selective distribution of upstream binding factor in rRNA transcription.Molecular biology of the cell · 1997Article
- In vivo evidence that TATA-binding protein/SL1 colocalizes with UBF and RNA polymerase I when rRNA synthesis is either active or inactive.The Journal of cell biology · 1996Article
- A possible mechanism for the inhibition of ribosomal RNA gene transcription during mitosis.The Journal of cell biology · 1995Article
- Number of nucleoli and coiled bodies and distribution of fibrillar centres in differentiating Purkinje neurons of chick and rat cerebellum.Anatomy and embryology · 1995Article
- Nuclear spreads: I. Visualization of bipartite ribosomal RNA domains.The Journal of cell biology · 1995Article
- The nucleolus.Anatomy and embryology · 1993Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The three-dimensional (3-D) organization of rDNA-containing chromatin and the set of protein markers of active ribosomal genes, the Ag-NOR proteins, were investigated by confocal laser scanning microscopy (CLSM). The rDNA genes of marsupial cells (PtK1) were mapped using biotinylated DNA probes for 45S rDNA sequences and the Ag-NOR protein distribution was revealed by specific Ag-NOR staining. We used PtK1 cells because each nucleolus possesses only one nucleolar organizer region (NOR). In metaphase chromosomes, nonisotopic in situ hybridization demonstrated the presence of rDNA in the secondary constriction of the X chromosomes with an axial distribution and also lateral expansions. 3-D reconstruction of the Ag-NOR protein signals revealed the presence of these proteins in the secondary constriction where they formed a crescent-shaped structure around the axial chromatin pedicule. The organization of the secondary constriction in PtK1 chromosomes is discussed. During interphase, nonisotopic in situ hybridization in intact cell monolayers and isolated nuclei showed the rDNA genes distributed as intense fluorescent spots linked by weak signals in the inner regions of the nucleoli. We conclude that the rDNA is not homogeneously distributed in the internal regions of the nucleoli. In the same nucleolar regions, the Ag-NOR proteins were revealed as granules linked by thin filaments. These images indicate similar 3-D distributions for rDNA probes and Ag-NOR proteins. The beaded organization of the transcriptional regions in the nucleoli is discussed.
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