ArticleNucleic acids research1993
Cloning of two novel forms of human acidic fibroblast growth factor (aFGF) mRNA.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 38 citations in OpenAlex.
- A Tri-fusion Reporter Mouse Reveals Tissue-Specific FGF1B Promoter Activity in vivo.Scientific reports · 2019Article
- Activation of heat shock response augments fibroblast growth factor-1 expression in wounded lung epithelium.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Regulation of FGF1 gene promoter through transcription factor RFX1.The Journal of biological chemistry · 2010Article
- Steroid-regulated growth of DDT1MF-2 cells is profoundly influenced by culture conditions.In vitro cellular & developmental biology. Animal · 1995Article
- Molecular and biochemical characterization of a Coccidioides immitis-specific antigen.Infection and immunity · 1995Article
- Human fibroblast growth factor 1 gene expression in vascular smooth muscle cells is modulated via an alternate promoter in response to serum and phorbol ester.Nucleic acids research · 1995Article
- Regulation of prostate growth by fibroblast growth factors.World journal of urology · 1995Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
We have previously isolated two different aFGF cDNA clones from kidney and brain. The two corresponding mRNA, designated aFGF 1.A and 1.B, are the predominant species in kidney and brain, respectively. During the characterization of aFGF mRNA in glioblastoma cells, we demonstrated that aFGF mRNA in U1242MG and D65MG glioblastoma cells contain 5'-untranslated sequences different from those of 1.A and 1.B. Through a strategy combining chromosome walking, identification and sequencing of evolutionarily conserved DNA regions, and a reverse transcription and polymerase chain reaction (RT-PCR)-based assay for RNA expression, we have isolated two novel aFGF cDNA clones. The cDNA clone representing aFGF mRNA 1.C was isolated from U1242MG cells; another aFGF cDNA, designated 1.D, was isolated from D65MG cells. Promoter 1C has extensive sequence homology to the hamster aFGF gene promoter that was shown to respond to testosterone stimulation by chloramphenicol acetyltransferase reporter gene assays. Using RT-PCR, we showed that normal, benign and cancerous prostate tissues do not express aFGF 1.C mRNA. In contrast, a prostate carcinoma cell line (PC-3) expresses 1.C mRNA. RT-PCR using 1.D-specific primers showed that kidney, brain and prostate do not express 1.D mRNA even though kidney and brain are the most abundant source for aFGF protein. RNase protection analysis further showed that 1.D mRNA is the predominant aFGF transcript in D65MG glioblastoma cells and in NFF-6 neonatal foreskin fibroblast cells. The genomic DNA corresponding to these two cDNA clones and the 5'-flanking regions were also isolated and their sequences determined. These DNA clones will provide important reagents for studying the regulatory elements of aFGF gene expression.
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