ArticleMolecular and cellular biology1993
cis regulation of the keratin 18 gene in transgenic mice.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
- Differential sensitivity of mouse epithelial tissues to the polyomavirus middle T oncogene.The American journal of pathology · 2006Article
- Exploiting the keratin 17 gene promoter to visualize live cells in epithelial appendages of mice.Molecular and cellular biology · 2005Article
- Transcriptional deregulation of the keratin 18 gene in human colon carcinoma cells results from an altered acetylation mechanism.Nucleic acids research · 2002Article
- Article
- Regulation of keratin and integrin gene expression in cancer and drug resistance.Cytotechnology · 1998Article
- Defective trophoblast function in mice with a targeted mutation of Ets2.Genes & development · 1998Article
- Methylation of an ETS site in the intron enhancer of the keratin 18 gene participates in tissue-specific repression.Molecular and cellular biology · 1997Article
- Oncogenic regulation and function of keratins 8 and 18.Cancer metastasis reviews · 1996Review
- Contrasting effects of the SATB1 core nuclear matrix attachment region and flanking sequences of the keratin 18 gene in transgenic mice.Molecular biology of the cell · 1996Article
- The consensus sequence of a major Alu subfamily contains a functional retinoic acid response element.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- An Sp1 binding site and the minimal promoter contribute to overexpression of the cytokeratin 18 gene in tumorigenic clones relative to that in nontumorigenic clones of a human carcinoma cell line.Molecular and cellular biology · 1995Article
- AP-1, ETS, and transcriptional silencers regulate retinoic acid-dependent induction of keratin 18 in embryonic cells.Molecular and cellular biology · 1994Article
- Specific combinations of human serum albumin introns direct high level expression of albumin in transfected COS cells and in the milk of transgenic mice.Transgenic research · 1994Article
- Evolutionary selection against change in many Alu repeat sequences interspersed through primate genomes.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Eukaryotic transcription termination factor La mediates transcript release and facilitates reinitiation by RNA polymerase III.Molecular and cellular biology · 1994Article
- Oncogene activation of human keratin 18 transcription via the Ras signal transduction pathway.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Alu sequence involvement in transcriptional insulation of the keratin 18 gene in transgenic mice.Molecular and cellular biology · 1993Article
- Transcriptional insulation of the human keratin 18 gene in transgenic mice.Molecular and cellular biology · 1993Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The gene coding for human keratin 18 (K18), a type I intermediate filament protein found in a variety of simple epithelia, is regulated correctly in transgenic mice but is promiscuously expressed after direct transfection into cell culture lines. We have begun an investigation of the mechanisms responsible for the correct regulation of K18 with a comparison of the chromatin state of K18 in permissive and nonpermissive transgenic mouse tissues to identify seven expression-specific, DNase-hypersensitive sites that correlate with known or potential regulatory regions of the gene. Four of these sites are associated with the proximal promoter region and the first intron that has been implicated previously in the transcriptional control of K18. Two hypersensitive sites are associated with a conserved Alu repetitive sequence located immediately upstream of the proximal promoter elements. Transcription of this Alu element in a direction opposite that of K18 was correlated with K18 expression in transgenic tissues. The final hypersensitive site was mapped to exon 6. The potential importance of this region for the expression of K18 was supported by the results of transient expression of the gene and various deleted constructions. In addition, exon 6 and the intron 1 regulatory region were distinguished from the remainder of K18 by differential DNA methylation in expressing and nonexpressing tissues. The CpG-rich proximal promoter and first exon regions remain unmethylated in both permissive and nonpermissive tissues. These results suggest that DNA methylation is not the primary mechanism of control of the gene. An Alu RNA polymerase III transcription unit and exon 6 are implicated in regulation of K18.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.