ArticleMolecular and cellular biology1993
In vitro activation of the transcription factor gamma interferon activation factor by gamma interferon: evidence for a tyrosine phosphatase/kinase signaling cascade.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 63 citations in OpenAlex.
- Vaccinia virus blocks gamma interferon signal transduction: viral VH1 phosphatase reverses Stat1 activation.Journal of virology · 2001Article
- Shp-2 tyrosine phosphatase functions as a negative regulator of the interferon-stimulated Jak/STAT pathway.Molecular and cellular biology · 1999Article
- Enhancement of antiproliferative activity of gamma interferon by the specific inhibition of tyrosine dephosphorylation of Stat1.Molecular and cellular biology · 1996Article
- Leukemia inhibitory factor (LIF) stimulates proopiomelanocortin (POMC) expression in a corticotroph cell line. Role of STAT pathway.The Journal of clinical investigation · 1996Article
- Inhibition of alpha interferon but not gamma interferon signal transduction by phorbol esters is mediated by a tyrosine phosphatase.Molecular and cellular biology · 1996Article
- Differential regulation of the alpha/beta interferon-stimulated Jak/Stat pathway by the SH2 domain-containing tyrosine phosphatase SHPTP1.Molecular and cellular biology · 1995Article
- Ligand-dependent and -independent activation of the transcription factor gamma RF-1 in a cell-free system.The Biochemical journal · 1995Article
- Signal transduction and activation of gene transcription by interferons.Gene expression · 1995Review
- Class II transactivator (CIITA) is sufficient for the inducible expression of major histocompatibility complex class II genes.The Journal of experimental medicine · 1994Article
- Prolactin activates the interferon-regulated p91 transcription factor and the Jak2 kinase by tyrosine phosphorylation.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- The interleukin-6-activated acute-phase response factor is antigenically and functionally related to members of the signal transducer and activator of transcription (STAT) family.Molecular and cellular biology · 1994Article
- Ligand-induced IFN gamma receptor tyrosine phosphorylation couples the receptor to its signal transduction system (p91).The EMBO journal · 1994Article
- Mutant cell lines unresponsive to alpha/beta and gamma interferon are defective in tyrosine phosphorylation of ISGF-3 alpha components.Molecular and cellular biology · 1994Article
- Growth hormone and erythropoietin differentially activate DNA-binding proteins by tyrosine phosphorylation.Molecular and cellular biology · 1994Article
- The signalling pathways of interleukin-6 and gamma interferon converge by the activation of different transcription factors which bind to common responsive DNA elements.Molecular and cellular biology · 1994Article
- Human cancer cell lines express a negative transcriptional regulator of the interferon regulatory factor family of DNA binding proteins.Molecular and cellular biology · 1994Article
- A factor induced by differentiation signals in cells of the macrophage lineage binds to the gamma interferon activation site.Molecular and cellular biology · 1994Article
- A nuclear tyrosine phosphatase downregulates interferon-induced gene expression.Molecular and cellular biology · 1993Article
- In vitro activation of a transcription factor by gamma interferon requires a membrane-associated tyrosine kinase and is mimicked by vanadate.Molecular and cellular biology · 1993Article
- The response of gamma interferon activation factor is under developmental control in cells of the macrophage lineage.Molecular and cellular biology · 1993Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although it has been well documented that the biological activities of gamma interferon (IFN-gamma) are initiated through interaction with its cell surface receptor, the signal transduction mechanisms which mediate the effects of this cytokine have remained unclear. In order to facilitate a better understanding of IFN-gamma signaling, we have designed an assay using human fibroblast cell homogenates in which IFN-gamma activates the formation of the IFN-gamma activation factor (GAF) transcription complex. GAF mediates the rapid transcriptional activation of the guanylate-binding protein gene by IFN-gamma. Activation of GAF in homogenates required ATP, but not Ca2+ or GTP. Fractionation of homogenates indicated that both the pellet (18,000 x g) and the remaining cytoplasmic fraction were required for GAF activation by IFN-gamma. In intact cells and cell homogenates, the activation of GAF was prevented by the specific tyrosine kinase inhibitor genistein. Treatment of GAF-containing nuclear extracts with either monoclonal antiphosphotyrosine antibody or protein tyrosine phosphatase prevented the assembly of the transcription complex, indicating that its formation required phosphorylation of tyrosine residues. Furthermore, the tyrosine phosphatase inhibitors phenylarsine oxide and zinc chloride also inhibited GAF formation in vitro, but only if these agents were added to cell homogenates before IFN-gamma was added. The addition of either agent 5 min after IFN-gamma had no effect. These results provide the first evidence for an IFN-gamma-regulated tyrosine phosphatase/kinase signaling cascade that permits this cytokine to activate the transcription of an early-response gene.
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