ArticleThe Journal of cell biology1993
Do photobleached fluorescent microtubules move?: re-evaluation of fluorescence laser photobleaching both in vitro and in growing Xenopus axon.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 54 citations in OpenAlex.
- Photoactivatable fluorophores and techniques for biological imaging applications.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2012Review
- Neurofilaments form a highly stable stationary cytoskeleton after reaching a critical level in axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- A physical model of axonal elongation: force, viscosity, and adhesions govern the mode of outgrowth.Biophysical journal · 2008Article
- Live-cell imaging of slow axonal transport in cultured neurons.Methods in cell biology · 2003Article
- Rapid intermittent movement of axonal neurofilaments observed by fluorescence photobleaching.Molecular biology of the cell · 2001Article
- Glutamate slows axonal transport of neurofilaments in transfected neurons.The Journal of cell biology · 2000Article
- Reorganization and movement of microtubules in axonal growth cones and developing interstitial branches.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1999Article
- Slow transport of unpolymerized tubulin and polymerized neurofilament in the squid giant axon.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Slow axonal transport of neurofilament protein in cultured neurons.The Journal of cell biology · 1999Article
- Transport and turnover of microtubules in frog neurons depend on the pattern of axonal growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Tubulin transport in neurons.The Journal of cell biology · 1996Article
- Active transport of photoactivated tubulin molecules in growing axons revealed by a new electron microscopic analysis.The Journal of cell biology · 1996Article
- A composite model for establishing the microtubule arrays of the neuron.Molecular neurobiology · 1996Review
- Differential dynamics of neurofilament-H protein and neurofilament-L protein in neurons.The Journal of cell biology · 1994Article
- Phosphorylation on carboxyl terminus domains of neurofilament proteins in retinal ganglion cell neurons in vivo: influences on regional neurofilament accumulation, interneurofilament spacing, and axon caliber.The Journal of cell biology · 1994Article
- A new perspective on microtubules and axon growth.The Journal of cell biology · 1993Review
- Time series characterization of simulated microtubule dynamics in the nerve growth cone.Annals of biomedical engineeringArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously documented differences in the behavior of microtubules in growing axons of two types of neurons, adult mouse sensory neurons and Xenopus embryonal spinal cord neurons. Namely, the bulk of microtubules was stationary in mouse sensory neurons both by the method of photoactivation of caged-fluorescein-labeled tubulin and photobleaching of fluorescein-labeled tubulin, but the bulk of microtubules did translocate anterogradely by the method of photoactivation. Although these results indicated that the stationary nature of photobleached microtubules in mouse neurons is not an artifact derived from the high levels of energy required for the procedure, it has not yet been settled whether the photobleaching method can detect the movement of microtubules properly. Here we report photobleaching experiments on growing axons of Xenopus embryonal neurons. Anterograde movement of photobleached microtubules was observed at a frequency and translocation rate similar to the values determined by the method of photoactivation. Our results suggest that, under appropriate conditions, the photobleaching method is able to reveal the behavior of microtubules as accurately as the photoactivation method.
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