ReviewThe Histochemical journal1993
Practical suggestions for successful immunoenzyme double-staining experiments.
Review in The Histochemical journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Galactosidase-catalyzed fluorescence amplification method (GAFAM): sensitive fluorescent immunohistochemistry using novel fluorogenic β-galactosidase substrates and its application in multiplex immunostaining.Histochemistry and cell biology · 2023Article
- How to distinguish between different cell lineages sharing common markers using combinations of double in-situ-hybridization and immunostaining in avian embryos: CXCR4-positive mesodermal and neural crest-derived cells.Histochemistry and cell biology · 2021Article
- Post-diaminobenzidine Treatments for Double Stainings: Extension of Sulfide-Silver-Gold Intensification for Light and Fluorescent Microscopy.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
- Neopterin and Cardiovascular Events Following Coronary Stent Implantation in Patients with Stable Angina Pectoris.Journal of atherosclerosis and thrombosis · 2018Article
- Article
- Phenotypic change and accumulation of smooth muscle cells in strictures in Crohn's disease: relevance to local angiotensin II system.Journal of gastroenterology · 2010Article
- Multiple immunoenzyme staining: methods and visualizations for the observation with spectral imaging.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2008Article
- Enhanced expressions of endothelin-converting enzyme and endothelin receptors in human colonic tissues of Crohn's disease.Journal of clinical biochemistry and nutrition · 2008Article
- Neopterin is associated with plaque inflammation and destabilisation in human coronary atherosclerotic lesions.Heart (British Cardiac Society) · 2007Article
- Immunohistochemical demonstration of histone H1(0) in human breast carcinoma.Histochemistry and cell biology · 2005Article
- Interferon-gamma reduces melanosomal antigen expression and recognition of melanoma cells by cytotoxic T cells.The American journal of pathology · 2002Article
- Role of endothelial cell denudation and smooth muscle cell dedifferentiation in neointimal formation of human vein grafts after coronary artery bypass grafting: therapeutic implications.Heart (British Cardiac Society) · 2000Article
- Double staining of intracellular cytokine proteins and T-lymphocyte subsets. Evaluation of the method in blood and bronchoalveolar lavage fluid.The Histochemical journal · 2000Article
- Immunohistological analysis of in situ expression of mycobacterial antigens in skin lesions of leprosy patients across the histopathological spectrum. Association of Mycobacterial lipoarabinomannan (LAM) and Mycobacterium leprae phenolic glycolipid-I (PGL-I) with leprosy reactions.The American journal of pathology · 1999Article
- Enrichment of human bone marrow mononuclear phagocytes and characterization of macrophage subpopulations by immunoenzymatic double staining.The Histochemical journal · 1998Article
- Preferential expression of the mucosal homing receptor integrin alpha 4 beta 7 in gastrointestinal non-Hodgkin's lymphomas.The American journal of pathology · 1997Article
- The use of enhanced polymer one-step staining reagents for immunoenzyme double-labelling.The Histochemical journal · 1996Article
- Kupffer cells and pit cells are not effective in the defense against experimentally induced colon carcinoma metastasis in rat liver.Clinical & experimental metastasis · 1996Article
- A combined staining method for argyrophilic nucleolar organizer regions and for glial fibrillary acidic protein in astrocytes of human brain.The Histochemical journal · 1996Article
- Expression of platelet derived growth factor B chain and beta receptor in human coronary arteries after percutaneous transluminal coronary angioplasty: an immunohistochemical study.Heart (British Cardiac Society) · 1996Article
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3 authors.
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Abstract
Many methodologies exist to perform an immunoenzyme double staining. Hence, the practical problem arises as to which of these methods is optimal for one's own experimental design. A process of selection is described which is derived from our own practical experience. First, a general strategy is outlined for the handling of tissue sections to be used for multiple staining methods. Secondly, the selection of an appropriate immunoenzyme double-staining concept is made using a flow chart. Thereafter we give criteria for the definitive selection of an immunoenzyme double-staining protocol based on the characteristics of the tissue or cell type under study. Particular attention is given to the selection of appropriate detection systems, applying enzymes or gold particles, and good contrasting colour combinations. The problems of visualizing co-localization using immunoenzyme double staining are dealt with, and suggestions are made to adapt the method, if necessary, in order to optimize it.
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