ArticleProceedings of the National Academy of Sciences of the United States of America1993
Expression of a constitutively active erythropoietin receptor in primary hematopoietic progenitors abrogates erythropoietin dependence and enhances erythroid colony-forming unit, erythroid burst-forming unit, and granulocyte/macrophage progenitor growth.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 61 citations in OpenAlex.
- Unpaired Extracellular Cysteine Mutations of CSF3R Mediate Gain or Loss of Function.Cancer research · 2017Article
- JAK2 V617F stimulates proliferation of erythropoietin-dependent erythroid progenitors and delays their differentiation by activating Stat1 and other nonerythroid signaling pathways.Experimental hematology · 2016Article
- Article
- The erythropoietin receptor: molecular structure and hematopoietic signaling pathways.Journal of investigative medicine : the official publication of the American Federation for Clinical Research · 2011Review
- Erythropoietin blockade inhibits the induction of tumor angiogenesis and progression.PloS one · 2007Article
- Hematopoietic growth factors, signaling and the chronic myeloproliferative disorders.Cytokine & growth factor reviews · 2006Review
- Characterization of purified intraembryonic hematopoietic stem cells as a tool to define their site of origin.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Enforced granulocyte/macrophage colony-stimulating factor signals do not support lymphopoiesis, but instruct lymphoid to myelomonocytic lineage conversion.The Journal of experimental medicine · 2003Article
- Erythropoietin receptors that signal through Stat5 or Stat3 support fetal liver and adult erythropoiesis: lack of specificity of stat signals during red blood cell development.Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research · 2000Article
- BCR-ABL and v-SRC tyrosine kinase oncoproteins support normal erythroid development in erythropoietin receptor-deficient progenitor cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Oligomerization and scaffolding functions of the erythropoietin receptor cytoplasmic tail.The Journal of biological chemistry · 1999Article
- Dysregulated hematopoiesis and a progressive neurological disorder induced by expression of an activated form of the human common beta chain in transgenic mice.The Journal of clinical investigation · 1998Article
- Absence of cytokine receptor-dependent specificity in red blood cell differentiation in vivo.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Control of hematopoietic differentiation: lack of specificity in signaling by cytokine receptors.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Cooperation of Spi-1/PU.1 with an activated erythropoietin receptor inhibits apoptosis and Epo-dependent differentiation in primary erythroblasts and induces their Kit ligand-dependent proliferation.The EMBO journal · 1997Article
- Article
- Constitutive activation of a variant of the env-mpl oncogene product by disulfide-linked homodimerization.Journal of virology · 1995Article
- Expression of an activated erythropoietin or a colony-stimulating factor 1 receptor by pluripotent progenitors enhances colony formation but does not induce differentiation.Proceedings of the National Academy of Sciences of the United States of America · 1994Article
- Murine pluripotent hematopoietic progenitors constitutively expressing a normal erythropoietin receptor proliferate in response to erythropoietin without preferential erythroid cell differentiation.Molecular and cellular biology · 1994Article
- Activation and inhibition of erythropoietin receptor function: role of receptor dimerization.Molecular and cellular biology · 1994Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We tested the ability of a constitutively activated erythropoietin receptor [EpoR(R129C)] to alter the growth requirements of primary hematopoietic precursors that terminally differentiate in culture. Two recombinant retroviruses expressing EpoR(R129C), spleen focus-forming virus (SFFVc-EpoR) and myeloproliferative sarcoma virus (MPSVcEpoR), were used to infect fetal liver cells that served as a source of hematopoietic progenitors. Methylcellulose cultures were incubated in the absence of any added growth factors or in combination with selected growth factors. EpoR(R129C) completely abrogated the Epo requirement of erythroid colony-forming units to form erythrocytes after 2-5 days in culture and did not interfere with the differentiation program of these cells. In the absence of added growth factors EpoR(R129C) did not enhance erythroid burst-forming unit development. In contrast to experiments in heterologous cell lines, EpoR(R129C) did not render progenitor cells independent of interleukin 3 or granulocyte/macrophage colony-stimulating factor (GM-CSF). However, when progenitors were cultured with added steel factor, but not with interleukin 3 or GM-CSF, EpoR(R129C) augmented the growth and differentiation of erythroid bursts, mixed erythroid/myeloid, and granulocyte/macrophage (GM) colonies. Furthermore, both viruses were capable of expressing EpoR(R129C) in erythroid, mixed erythroid/myeloid, and GM colonies. Thus an aberrantly expressed and constitutively activated EpoR can stimulate proliferation of some GM progenitors. The ability of EpoR(R129C) to abrogate the Epo requirement of primary hematopoietic cells, but not the requirement for other cytokines, is consistent with the induction of erythroblastosis in vivo.
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