ArticleThe EMBO journal1993
Nuclear uptake control of NF-kappa B by MAD-3, an I kappa B protein present in the nucleus.
Article in The EMBO journal, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 120 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
120 citing papers in PubMed, 319 citations in OpenAlex.
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- Regulating Phase Transition in Neurodegenerative Diseases by Nuclear Import Receptors.Biology · 2022Review
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- Signaling Crosstalk Mechanisms That May Fine-Tune Pathogen-Responsive NFκB.Frontiers in immunology · 2019Review
- 14-3-3z sequesters cytosolic T-bet, upregulating IL-13 levels in TThe Journal of allergy and clinical immunology · 2018Article
- Transcription Factors in the Cellular Response to Charged Particle Exposure.Frontiers in oncology · 2016Review
- The ubiquitin ligase HERC3 attenuates NF-κB-dependent transcription independently of its enzymatic activity by delivering the RelA subunit for degradation.Nucleic acids research · 2015Article
- The presence of nuclear cactus in the early Drosophila embryo may extend the dynamic range of the dorsal gradient.PLoS computational biology · 2015Article
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- NF-κB inhibitor targeted to activated endothelium demonstrates a critical role of endothelial NF-κB in immune-mediated diseases.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Differential localization of T-bet and Eomes in CD8 T cell memory populations.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- An adeno-associated virus-based intracellular sensor of pathological nuclear factor-κB activation for disease-inducible gene transfer.PloS one · 2013Article
- Correlation of Notch1, pAKT and nuclear NF-κB expression in triple negative breast cancer.American journal of cancer research · 2013Article
- Novel signaling mechanisms of intracellular angiotensin II-induced NHE3 expression and activation in mouse proximal tubule cells.American journal of physiology. Renal physiology · 2012Article
- Bone morphogenetic protein signaling in vascular disease: anti-inflammatory action through myocardin-related transcription factor A.The Journal of biological chemistry · 2012Article
- KrasG12D-induced IKK2/β/NF-κB activation by IL-1α and p62 feedforward loops is required for development of pancreatic ductal adenocarcinoma.Cancer cell · 2012Article
- Article
60 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
I kappa B proteins specifically inhibit the DNA binding of NF-kappa B/Rel transcription factors. An additional role as inhibitors of nuclear uptake was supposed by subcellular fractionation and enucleation experiments. Using indirect immunofluorescence labeling of cells, we show here that the DNA-binding p50 and p65 subunits of NF-kappa B, as well as the I kappa B protein MAD-3, all occur in the nucleus when overexpressed on their own. Nuclear uptake of p65 and, to a lesser extent of p50, was, however, suppressed when MAD-3 was coexpressed. Likewise, nuclear uptake of MAD-3 was blocked by overexpressed p65 or p50. This directly demonstrates that I kappa B is a nuclear uptake regulatory protein and that the various subunits of NF-kappa B can mutually control their access to the nucleus. In the presence of MAD-3, antibodies specific for peptides overlapping the nuclear location signal (NLS) sequences of p65 and p50 could not recognize their epitopes on NF-kappa B, suggesting that the I kappa B protein rendered the signals inaccessible for NLS receptors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.