ArticleThe Journal of cell biology1993
Ectopic synthesis of epidermal cytokeratins in pancreatic islet cells of transgenic mice interferes with cytoskeletal order and insulin production.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 69 citations in OpenAlex.
- Altered keratinocyte differentiation is an early driver of keratin mutation-based palmoplantar keratoderma.Human molecular genetics · 2019Article
- Differential Evolution of the Epidermal Keratin Cytoskeleton in Terrestrial and Aquatic Mammals.Molecular biology and evolution · 2019Article
- Decreased levels of keratin 8 sensitize mice to streptozotocin-induced diabetes.Acta physiologica (Oxford, England) · 2018Article
- Keratins Are Going Nuclear.Developmental cell · 2016Article
- A Drosophila Model of Epidermolysis Bullosa Simplex.The Journal of investigative dermatology · 2015Article
- Keratin 8 modulates β-cell stress responses and normoglycaemia.Journal of cell science · 2013Article
- Cytoskeleton in motion: the dynamics of keratin intermediate filaments in epithelia.The Journal of cell biology · 2011Review
- Intermediate filaments: a role in epithelial polarity.Experimental cell research · 2007Review
- Keratin 8 sequence variants in patients with pancreatitis and pancreatic cancer.Journal of molecular medicine (Berlin, Germany) · 2006Article
- Pairwise assembly determines the intrinsic potential for self-organization and mechanical properties of keratin filaments.Molecular biology of the cell · 2002Article
- Modulation of cell proliferation by cytokeratins K10 and K16.Molecular and cellular biology · 1999Article
- Functional differences between keratins of stratified and simple epithelia.The Journal of cell biology · 1998Article
- Directed expression of keratin 16 to the progenitor basal cells of transgenic mouse skin delays skin maturation.The Journal of cell biology · 1998Article
- Lessons from keratin 18 knockout mice: formation of novel keratin filaments, secondary loss of keratin 7 and accumulation of liver-specific keratin 8-positive aggregates.The Journal of cell biology · 1998Article
- Topogenesis of a nucleolar protein: determination of molecular segments directing nucleolar association.Molecular biology of the cell · 1997Article
- A transgenic mouse model with an inducible skin blistering disease phenotype.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Susceptibility to hepatotoxicity in transgenic mice that express a dominant-negative human keratin 18 mutant.The Journal of clinical investigation · 1996Article
- Gene targeting at the mouse cytokeratin 10 locus: severe skin fragility and changes of cytokeratin expression in the epidermis.The Journal of cell biology · 1996Article
- Onset of re-epithelialization after skin injury correlates with a reorganization of keratin filaments in wound edge keratinocytes: defining a potential role for keratin 16.The Journal of cell biology · 1996Article
- Chronic hepatitis, hepatocyte fragility, and increased soluble phosphoglycokeratins in transgenic mice expressing a keratin 18 conserved arginine mutant.The Journal of cell biology · 1995Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The members of the multigene family of intermediate filament (IF) proteins are expressed in various combinations and amounts that are specific for a given pathway or state of differentiation. Previous experiments in which the cell type-specific IF cytoskeleton was altered by introducing foreign IF proteins into cultured cells or certain tissues of transgenic animals have shown a remarkable tolerance, without detectable interference with cell functions. To examine the importance of the cell type-specific cytokeratin (CK) IF pattern, we have studied the ectopic expression of CK genes in different epithelia of transgenic mice. Here we report changes observed in the beta cells of pancreatic islets expressing the genes for human epidermal CKs 1 and/or 10 brought under control of the rat insulin promoter. Both genes were efficiently expressed, resulting in the appearance of numerous and massive bundles of aggregated IFs, resembling those of epidermal keratinocytes. While the synthesis of epidermal CK 10 was readily accommodated and compatible with cell function, mice expressing CK 1 in their beta cells, alone or in combination with CK 10, developed a special form of diabetes characterized by a drastic reduction of insulin-secretory vesicles and of insulin-and CK 1-producing cells. In many CK 1-producing cells, accumulations of fibrous or granular material containing CK 1 were also seen in the nucleus. This demonstration of functional importance of the specific CK-complement in an epithelial cell indicates a contribution of cell type-specific factors to cytoplasmic IF compartmentalization and that the specific CK complement can be crucial for functions and longevity of a given kind of epithelium.
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