ArticleThe Journal of cell biology1993
GTP gamma S inhibits organelle transport along axonal microtubules.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
- Transcriptomic Dynamics of Active and Inactive States of Rho GTPase MoRho3 inJournal of fungi (Basel, Switzerland) · 2022Article
- Microtubules: Evolving roles and critical cellular interactions.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- The axonal transport of mitochondria.Journal of cell science · 2012Review
- Mitochondrial configurations in peripheral nerve suggest differential ATP production.Journal of structural biology · 2011Article
- APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- The amino terminus of tau inhibits kinesin-dependent axonal transport: implications for filament toxicity.Journal of neuroscience research · 2009Article
- UNC-51/ATG1 kinase regulates axonal transport by mediating motor-cargo assembly.Genes & development · 2008Article
- Spatiotemporal regulation of ATP and Ca2+ dynamics in vertebrate rod and cone ribbon synapses.Molecular vision · 2007Article
- 1-Methyl-4-phenylpyridinium affects fast axonal transport by activation of caspase and protein kinase C.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- The axonal transport of mitochondria.Journal of cell science · 2005Review
- Review
- A novel CDK5-dependent pathway for regulating GSK3 activity and kinesin-driven motility in neurons.The EMBO journal · 2004Article
- Glycogen synthase kinase 3 phosphorylates kinesin light chains and negatively regulates kinesin-based motility.The EMBO journal · 2002Article
- Fast axonal transport misregulation and Alzheimer's disease.Neuromolecular medicine · 2002Review
- Release of kinesin from vesicles by hsc70 and regulation of fast axonal transport.Molecular biology of the cell · 2000Article
- Axonal membrane proteins are transported in distinct carriers: a two-color video microscopy study in cultured hippocampal neurons.Molecular biology of the cell · 2000Article
- In vitro reconstitution of microtubule plus end-directed, GTPgammaS-sensitive motility of Golgi membranes.Molecular biology of the cell · 1998Article
- A role for cyclin-dependent kinase(s) in the modulation of fast anterograde axonal transport: effects defined by olomoucine and the APC tumor suppressor protein.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Effects of exogenous triiodothyronine on fast axonal transport during tadpole metamorphosis.Neurochemical research · 1996Article
- A phosphatidylinositol 4,5-bisphosphate-sensitive casein kinase I alpha associates with synaptic vesicles and phosphorylates a subset of vesicle proteins.The Journal of cell biology · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Movements of membrane-bounded organelles through cytoplasm frequently occur along microtubules, as in the neuron-specific case of fast axonal transport. To shed light on how microtubule-based organelle motility is regulated, pharmacological probes for GTP-binding proteins, or protein kinases or phosphatases were perfused into axoplasm extruded from squid (Loligo pealei) giant axons, and effects on fast axonal transport were monitored by quantitative video-enhanced light microscopy. GTP gamma S caused concentration-dependent and time-dependent declines in organelle transport velocities. GDP beta S was a less potent inhibitor. Excess GTP, but not GDP, masked the effects of coperfused GTP gamma S. The effects of GTP gamma S on transport were not mimicked by broad spectrum inhibitors of protein kinases (K-252a) or phosphatases (microcystin LR and okadaic acid), or as shown earlier, by ATP gamma S. Therefore, suppression of organelle motility by GTP gamma S was guanine nucleotide-specific and evidently did not involve irreversible transfer of thiophosphate groups to protein. Instead, the data imply that organelle transport in the axon is modulated by cycles of GTP hydrolysis and nucleotide exchange by one or more GTP-binding proteins. Fast axonal transport was not perturbed by AlF4-, indicating that the GTP gamma S-sensitive factors do not include heterotrimeric G-proteins. Potential axoplasmic targets of GTP gamma S include dynamin and multiple small GTP-binding proteins, which were shown to be present in squid axoplasm. These collective findings suggest a novel strategy for regulating microtubule-based organelle transport and a new role for GTP-binding proteins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.