ArticleThe Journal of cell biology1993
A role of tyrosine phosphorylation in the formation of acetylcholine receptor clusters induced by electric fields in cultured Xenopus muscle cells.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 54 citations in OpenAlex.
- Protein Tyrosine Phosphatase Receptor Type R (PTPRR) Reduces AChR Clustering by Dephosphorylating MuSK.Disease markers · 2022Article
- Nerve, Muscle, and Synaptogenesis.Cells · 2019Review
- GSK-3β is essential for physiological electric field-directed Golgi polarization and optimal electrotaxis.Cellular and molecular life sciences : CMLS · 2011Article
- Article
- Specific agrin isoforms induce cAMP response element binding protein phosphorylation in hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- A role of tyrosine phosphatase in acetylcholine receptor cluster dispersal and formation.The Journal of cell biology · 1998Article
- Intercellular communication that mediates formation of the neuromuscular junction.Molecular neurobiology · 1997Review
- Common molecular mechanisms in field- and agrin-induced acetylcholine receptor clustering.Cellular and molecular neurobiology · 1997Article
- Modulation and selection of neurotransmitter responses during synapse formation between identified leech neurons.Cellular and molecular neurobiology · 1996Article
- Signaling from Neural Impulses to Genes.The Neuroscientist : a review journal bringing neurobiology, neurology and psychiatry · 1996Article
- Density and diffusion limited aggregation in membranes.Bulletin of mathematical biology · 1995Article
- Tyrosine phosphorylation during synapse formation between identified leech neurons.The Journal of physiology · 1995Article
- Distribution of alpha-dystroglycan during embryonic nerve-muscle synaptogenesis.The Journal of cell biology · 1995Article
- Staurosporine inhibits agrin-induced acetylcholine receptor phosphorylation and aggregation.The Journal of cell biology · 1994Article
- Tyrosine phosphorylation and acetylcholine receptor cluster formation in cultured Xenopus muscle cells.The Journal of cell biology · 1993Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
During the development of the neuromuscular junction, acetylcholine receptors (AChRs) become clustered in the postsynaptic membrane in response to innervation. In vitro, several non-neuronal stimuli can also induce the formation of AChR clusters. DC electric field (E field) is one of them. When cultured Xenopus muscle cells are exposed to an E field of 5-10 V/cm, AChRs become clustered along the cathode-facing edge of the cells within 2 h. Recent studies have suggested the involvement of tyrosine kinase activation in the action of several AChR clustering stimuli, including nerve, polymer beads, and agrin. We thus examined the role of tyrosine phosphorylation in E field-induced AChR clustering. An antibody against phosphotyrosine (PY) was used to examine the localization of PY-containing proteins in E field-treated muscle cells. We found that anti-PY staining was colocalized with AChR clusters along the cathodal edge of the cells. In fact, cathodal PY staining could be detected before the first appearance of AChR clusters. When cultures were subjected to E fields in the presence of a tyrosine kinase inhibitor, tyrphostin RG-50864, cathodal AChR clustering was abolished with a half maximal inhibitory dosage of 50 microM. An inactive form of tyrphostin (RG-50862) had no effect on the field-induced clustering. These data suggest that the activation of tyrosine kinases is an essential step in E field-induced AChR clustering. Thus, the actions of several disparate stimuli for AChR clustering seem to converge to a common signal transduction mechanism based on tyrosine phosphorylation at the molecular level.
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