ArticleProceedings of the National Academy of Sciences of the United States of America1995
Single-headed myosin II acts as a dominant negative mutation in Dictyostelium.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- A cross-tissue physicochemical causal chain underlying vertebrate mandibular morphogenesis.Science advances · 2026Article
- A theoretical model of cytokinesis implicates feedback between membrane curvature and cytoskeletal organization in asymmetric cytokinetic furrowing.Molecular biology of the cell · 2016Article
- Myosin heavy chain kinases play essential roles in Ca2+, but not cAMP, chemotaxis and the natural aggregation of Dictyostelium discoideum.Journal of cell science · 2012Article
- Article
- A novel guanine nucleotide exchange factor MyoGEF is required for cytokinesis.Cell cycle (Georgetown, Tex.) · 2006Article
- Dictyostelium and Acanthamoeba myosin II assembly domains go to the cleavage furrow of Dictyostelium myosin II-null cells.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Mutations in the relay loop region result in dominant-negative inhibition of myosin II function in Dictyostelium.EMBO reports · 2002Article
- Two heads of myosin are better than one for generating force and motion.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Assembly of thick filaments and myofibrils occurs in the absence of the myosin head.The EMBO journal · 1999Article
- Cooperativity between two heads of dictyostelium myosin II in in vitro motility and ATP hydrolysis.Biophysical journal · 1999Article
- Heterotrimeric kinesin II is the microtubule motor protein responsible for pigment dispersion in Xenopus melanophores.The Journal of cell biology · 1998Article
- Involvement of an actomyosin contractile ring in Saccharomyces cerevisiae cytokinesis.The Journal of cell biology · 1998Article
- Myosin heavy chain phosphorylation sites regulate myosin localization during cytokinesis in live cells.Molecular biology of the cell · 1997Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conventional myosin II is an essential protein for cytokinesis, capping of cell surface receptors, and development of Dictyostelium cells. Myosin II also plays an important role in the polarization and movement of cells. All conventional myosins are double-headed molecules but the significance of this structure is not understood since single-headed myosin II can produce movement and force in vitro. We found that expression of the tail portion of myosin II in Dictyostelium led to the formation of single-headed myosin II in vivo. The resultant cells contain an approximately equal ratio of double- and single-headed myosin II molecules. Surprisingly, these cells were completely blocked in cytokinesis and capping of concanavalin A receptors although development into fruiting bodies was not impaired. We found that this phenotype is not due to defects in myosin light chain phosphorylation. These results show that single-headed myosin II cannot function properly in vivo and that it acts as a dominant negative mutation for myosin II function. These results suggest the possibility that cooperativity of myosin II heads is critical for force production in vivo.
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