ArticleJournal of virology1995
An internal ribosomal entry signal in the rat VL30 region of the Harvey murine sarcoma virus leader and its use in dicistronic retroviral vectors.
Article in Journal of virology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Retrospective on the all-in-one retroviral nucleocapsid protein.Virus research · 2014Review
- Functional and structural analysis of the internal ribosome entry site present in the mRNA of natural variants of the HIV-1.PloS one · 2012Article
- Article
- Mechanisms employed by retroviruses to exploit host factors for translational control of a complicated proteome.Retrovirology · 2009Review
- Translational control of retroviruses.Nature reviews. Microbiology · 2007Review
- RNA helicase A interacts with divergent lymphotropic retroviruses and promotes translation of human T-cell leukemia virus type 1.Nucleic acids research · 2007Article
- Searching for IRES.RNA (New York, N.Y.) · 2006Review
- Characterization of two distinct RNA domains that regulate translation of the Drosophila gypsy retroelement.RNA (New York, N.Y.) · 2004Article
- The eukaryotic translation initiation factor 4GI is cleaved by different retroviral proteases.Journal of virology · 2003Article
- The leader of human immunodeficiency virus type 1 genomic RNA harbors an internal ribosome entry segment that is active during the G2/M phase of the cell cycle.Journal of virology · 2003Article
- Destiny of unspliced retroviral RNA: ribosome and/or virion?Journal of virology · 2002Review
- Viral strategies of translation initiation: ribosomal shunt and reinitiation.Progress in nucleic acid research and molecular biology · 2002Review
- The human immunodeficiency virus type 1 gag gene encodes an internal ribosome entry site.Journal of virology · 2001Article
- Rous sarcoma virus translation revisited: characterization of an internal ribosome entry segment in the 5' leader of the genomic RNA.Journal of virology · 2000Article
- Identification of an internal ribosome entry segment in the 5' region of the mouse VL30 retrotransposon and its use in the development of retroviral vectors.Journal of virology · 1999Article
- Regulated multicistronic expression technology for mammalian metabolic engineering.Cytotechnology · 1998Article
- Internal entry of ribosomes is directed by the 5' noncoding region of classical swine fever virus and is dependent on the presence of an RNA pseudoknot upstream of the initiation codon.Journal of virology · 1997Article
- RNA-protein interactions in regulation of picornavirus RNA translation.Microbiological reviews · 1996Review
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genetic organization of the 5' genomic RNA domain of the highly oncogenic Harvey murine sarcoma virus appears to be unusual in that a multifunctional untranslated leader precedes the v-ras oncogene. This 5' leader is 1,076 nucleotides in length and is formed of independent regions involved in key steps of the viral life cycle: (i) the Moloney murine leukemia virus 5' repeat, untranslated 5' region, and primer binding site sequences necessary for the first steps of proviral DNA synthesis, (ii) the virus-like 30S (VL30)-derived sequence containing a functional dimerization-packaging signal (E/DLS) directing viral RNA dimerization and packaging into MLV virions, and (iii) an Alu-like sequence preceding the 5' untranslated sequence of v-rasH which contains the initiation codon of the p21ras oncoprotein. These functional features, the unusual length of this leader (1,076 nucleotides), and the presence of stable secondary structures between the cap and the v-ras initiation codon might well cause a premature stop of the scanning ribosomes and thus inhibit v-ras translation. In order to understand how Harvey murine sarcoma virus achieves a high level of expression of the ras oncogene, we asked whether the rat VL30 sequence, 5' to v-ras, could contribute to an efficient synthesis of the ras oncoprotein. The implications of the VL30 sequence in the translation initiation of Ha-ras were investigated in the rabbit reticulocyte lysate system and in murine cells. Results show that the rat VL30 sequence allows a cap-independent translation of a downstream reporter gene both in vitro and in murine cells. Additional experiments performed with dicistronic neo.VL30.lacZ mRNAs indicate that the 5' VL30 sequence (positions 380 to 794) contains an internal ribosomal entry signal. This finding led us to construct a new dicistronic retroviral vector with which the rat VL30 sequence was able to direct the efficient expression of a 3' cistron and packaging of recombinant dicistronic RNA into murine leukemia virus virions.
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