ArticleThe EMBO journal1995
Identification of the cis-elements mediating the autogenous control of ribosomal protein L2 mRNA stability in yeast.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 32 citations in OpenAlex.
- Auto-regulatory feedback by RNA-binding proteins.Journal of molecular cell biology · 2019Review
- Ribosomal proteins as unrevealed caretakers for cellular stress and genomic instability.Oncotarget · 2014Review
- The yeast RPL9B gene is regulated by modulation between two modes of transcription termination.The EMBO journal · 2012Article
- Balanced production of ribosomal proteins.Gene · 2007Review
- Precision and functional specificity in mRNA decay.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Article
- Regulation of ribosome biosynthesis in Escherichia coli and Saccharomyces cerevisiae: diversity and common principles.Journal of bacteriology · 1999Review
- The cis acting sequences responsible for the differential decay of the unstable MFA2 and stable PGK1 transcripts in yeast include the context of the translational start codon.RNA (New York, N.Y.) · 1999Article
- ELAV proteins stabilize deadenylated intermediates in a novel in vitro mRNA deadenylation/degradation system.Genes & development · 1999Article
- Ribosomal protein S14 of Saccharomyces cerevisiae regulates its expression by binding to RPS14B pre-mRNA and to 18S rRNA.Molecular and cellular biology · 1999Article
- Posttranscriptional control of gene expression in yeast.Microbiology and molecular biology reviews : MMBR · 1998Review
- Processing of the intron-encoded U18 small nucleolar RNA in the yeast Saccharomyces cerevisiae relies on both exo- and endonucleolytic activities.Molecular and cellular biology · 1998Article
- Functional mapping of the translation-dependent instability element of yeast MATalpha1 mRNA.Molecular and cellular biology · 1996Article
- Mechanisms and control of mRNA turnover in Saccharomyces cerevisiae.Microbiological reviews · 1996Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ribosomal protein L2 (rpL2) of Saccharomyces cerevisiae regulates the accumulation of its own mRNA by a feedback mechanism. An RNA sequence is responsible for this control, initially characterized as a 360 nucleotide-long region, localized at the 5' end of the transcript. This region, fused to an unrelated coding sequence, is able to down-regulate the accumulation of the chimeric transcript when increased levels of rpL2 are induced in the cell. The target regulatory region also responds to regulation when inserted inside an intron, demonstrating that the control process can take place inside the nucleus. Deletion analysis from the 5' and 3' borders have restricted the responsive region to approximately 200 nt. The insertion of a poly-G cassette downstream of the regulatory region allowed the identification of truncated 3' cut-off poly(A)+ RNA molecules. The parallel identification of cut-off molecules containing the 5' portion of the transcript allowed us to deduce that the truncated products originate by endonucleolytic cleavage. Altogether, these results are consistent with a mechanism by which the presence of excess amounts of rpL2 in the cell triggers its own mRNA to a degradative pathway; this involves an initial endonucleolytic cleavage that is followed by exonucleolytic trimming. Such a regulatory mechanism shows interesting analogies with the translational regulation of r-proteins in Escherichia coli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.