ArticleThe EMBO journal1995
In vitro reconstitution of mammalian U2 and U5 snRNPs active in splicing: Sm proteins are functionally interchangeable and are essential for the formation of functional U2 and U5 snRNPs.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
49 citing papers in PubMed, 77 citations in OpenAlex.
- Loss of the RNA trimethylguanosine cap is compatible with nuclear accumulation of spliceosomal snRNAs but not pre-mRNA splicing or snRNA processing during animal development.PLoS genetics · 2020Article
- Composition and processing activity of a semi-recombinant holo U7 snRNP.Nucleic acids research · 2020Article
- The Sm-core mediates the retention of partially-assembled spliceosomal snRNPs in Cajal bodies until their full maturation.Nucleic acids research · 2018Article
- Regulation and function of CMTR1-dependent mRNA cap methylation.Wiley interdisciplinary reviews. RNA · 2017Review
- Pseudouridine synthase 1 deficient mice, a model for Mitochondrial Myopathy with Sideroblastic Anemia, exhibit muscle morphology and physiology alterations.Scientific reports · 2016Article
- Insight into the mechanisms and functions of spliceosomal snRNA pseudouridylation.World journal of biological chemistry · 2014Review
- In vitro reconstitution of yeast splicing with U4 snRNA reveals multiple roles for the 3' stem-loop.RNA (New York, N.Y.) · 2012Article
- Pseudouridine synthase 1: a site-specific synthase without strict sequence recognition requirements.Nucleic acids research · 2012Article
- An essential role for trimethylguanosine RNA caps in Saccharomyces cerevisiae meiosis and their requirement for splicing of SAE3 and PCH2 meiotic pre-mRNAs.Nucleic acids research · 2011Article
- Phosphorylation and the Cajal body: modification in search of function.Archives of biochemistry and biophysics · 2010Review
- Coilin-dependent snRNP assembly is essential for zebrafish embryogenesis.Nature structural & molecular biology · 2010Article
- RNA modifications: a mechanism that modulates gene expression.Methods in molecular biology (Clifton, N.J.) · 2010Review
- Cajal-body formation correlates with differential coilin phosphorylation in primary and transformed cell lines.Journal of cell science · 2009Article
- Combined in silico and experimental identification of the Pyrococcus abyssi H/ACA sRNAs and their target sites in ribosomal RNAs.Nucleic acids research · 2008Article
- Splicing-independent recruitment of spliceosomal small nuclear RNPs to nascent RNA polymerase II transcripts.The Journal of cell biology · 2007Article
- Functional spliceosomal A complexes can be assembled in vitro in the absence of a penta-snRNP.RNA (New York, N.Y.) · 2006Article
- Protein composition and electron microscopy structure of affinity-purified human spliceosomal B complexes isolated under physiological conditions.Molecular and cellular biology · 2006Article
- U2 snRNA-protein contacts in purified human 17S U2 snRNPs and in spliceosomal A and B complexes.Molecular and cellular biology · 2006Article
- Pseudouridine modification in Caenorhabditis elegans spliceosomal snRNAs: unique modifications are found in regions involved in snRNA-snRNA interactions.BMC molecular biology · 2005Article
- Cross-talk between snurportin1 subdomains.Molecular biology of the cell · 2005Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An in vitro reconstitution/splicing complementation system has been developed which has allowed the investigation of the role of mammalian U2 and U5 snRNP components in splicing. U2 or U5 snRNP cores are first reconstituted from purified native snRNP core proteins and snRNA in the absence of cellular extract and are subsequently added to splicing extracts depleted of either U2 or U5 snRNP. When snRNPs reconstituted with HeLa U2 or U5 snRNA were added to U2- or U5-depleted nuclear extract, splicing was complemented. Addition of naked snRNA, on the other hand, did not restore splicing, demonstrating that the core proteins are essential for both U2 and U5 snRNP functions in splicing. Hybrid U2 or U5 snRNPs, reconstituted with core proteins isolated from U1 or U2 snRNPs, were equally active in splicing complementation, indicating that the snRNP core proteins are functionally interchangeable. U5 snRNPs reconstituted from in vitro transcribed U5 snRNA restored splicing to a level identical to that observed with particles reconstituted from authentic HeLa U5 snRNA. In contrast, splicing could not be restored to U2-depleted extract by the addition of snRNPs reconstituted from synthetic U2 snRNA, suggesting that U2 snRNA base modifications are essential for U2 snRNP function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.