ArticleMolecular biology of the cell1995
Transgenic mice with reduced numbers of functional sperm receptors on their eggs reproduce normally.
Article in Molecular biology of the cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 37 citations in OpenAlex.
- Improving sperm selection strategies for assisted reproduction through closing the knowledge gap in sperm maturation mechanics.Human reproduction open · 2025Review
- Human Zona Pellucida Glycoproteins: Binding Characteristics With Human Spermatozoa and Induction of Acrosome Reaction.Frontiers in cell and developmental biology · 2021Review
- Mammalian egg coat modifications and the block to polyspermy.Molecular reproduction and development · 2020Review
- Glycan-Independent Gamete Recognition Triggers Egg Zinc Sparks and ZP2 Cleavage to Prevent Polyspermy.Developmental cell · 2018Article
- Egg Coat Proteins Across Metazoan Evolution.Current topics in developmental biology · 2018Review
- Gamete compatibility genes in mammals: candidates, applications and a potential path forward.Royal Society open science · 2017Review
- A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans.The Journal of cell biology · 2014Article
- The maternal to zygotic transition in mammals.Molecular aspects of medicine · 2013Review
- The molecular basis of gamete recognition in mice and humans.Molecular human reproduction · 2013Review
- Human sperm bind to the N-terminal domain of ZP2 in humanized zonae pellucidae in transgenic mice.The Journal of cell biology · 2012Article
- Human ZP4 is not sufficient for taxon-specific sperm recognition of the zona pellucida in transgenic mice.Reproduction (Cambridge, England) · 2011Article
- Inactivation of the Mgat1 gene in oocytes impairs oogenesis, but embryos lacking complex and hybrid N-glycans develop and implant.Molecular and cellular biology · 2004Article
- Zona pellucida glycoprotein mZP3 produced in milk of transgenic mice is active as a sperm receptor, but can be lethal to newborns.Transgenic research · 1999Article
- Inactivation of the mouse sperm receptor, mZP3, by site-directed mutagenesis of individual serine residues located at the combining site for sperm.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- Reduced fertility in mice deficient for the POU protein sperm-1.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Targeted disruption of the mZP3 gene results in production of eggs lacking a zona pellucida and infertility in female mice.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To initiate fertilization in mice, free-swimming sperm bind to mZP3, an approximately 83-kDa glycoprotein present in the ovulated egg zona pellucida (ZP). mZP3 is located periodically along the filaments that constitute the ZP. Sperm recognize and bind to specific oligosaccharides linked to one or more of five Ser residues clustered in the carboxy-terminal one-third of the mZP3 polypeptide. When all five Ser residues are converted to nonhydroxy amino acids by site-directed mutagenesis of the mZP3 gene, an inactive form of mZP3, called mZP3[ser], is secreted by embryonal carcinoma cells stably transfected with the mutated gene. Here, seven independent transgenic mouse lines were established that harbor the mutated mZP3 gene. In all lines, the mutant gene is expressed by growing oocytes and mZP3[ser] is synthesized, secreted, and incorporated into the ZP. Purified mZP3[ser] prepared from ovaries of transgenic mice, like mZP3[ser] from transfected embryonal carcinoma cells, is inactive in sperm binding assays in vitro. On the other hand, the presence of mZP3[ser] in the ZP does not significantly affect either the binding of sperm to ovulated eggs in vitro or the reproduction of the mice, i.e., the transgenic mice are fertile, breed at normal intervals, and produce litters of normal sizes. These results indicate that the number of functional sperm receptors in the ZP can be reduced by more than 50% without adversely affecting fertilization of eggs in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.