ArticleThe Journal of clinical investigation1995
The phosphorylation targets of p47phox, a subunit of the respiratory burst oxidase. Functions of the individual target serines as evaluated by site-directed mutagenesis.
Article in The Journal of clinical investigation, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 181 citations in OpenAlex.
- NADPH oxidases: redox regulation of cell homeostasis and disease.Physiological reviews · 2025Review
- Shear and Integrin Outside-In Signaling Activate NADPH-Oxidase 2 to Promote Platelet Activation.Arteriosclerosis, thrombosis, and vascular biology · 2021Article
- p47phox siRNA-Loaded PLGA Nanoparticles Suppress ROS/Oxidative Stress-Induced Chondrocyte Damage in Osteoarthritis.Polymers · 2020Article
- The Emerging Roles of Nicotinamide Adenine Dinucleotide Phosphate Oxidase 2 in Skeletal Muscle Redox Signaling and Metabolism.Antioxidants & redox signaling · 2019Review
- Redox Activation of Nox1 (NADPH Oxidase 1) Involves an Intermolecular Disulfide Bond Between Protein Disulfide Isomerase and p47Arteriosclerosis, thrombosis, and vascular biology · 2019Article
- A Role for MK2 in Enhancing Neutrophil-Derived ROS Production and Aggravating Liver Ischemia/Reperfusion Injury.Frontiers in immunology · 2018Article
- HIV Protease Inhibitor-Induced Cathepsin Modulation Alters Antigen Processing and Cross-Presentation.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- NOX Activation by Subunit Interaction and Underlying Mechanisms in Disease.Frontiers in cellular neuroscience · 2016Review
- Molecular insights of p47phox phosphorylation dynamics in the regulation of NADPH oxidase activation and superoxide production.The Journal of biological chemistry · 2014Article
- Assembly of NADPH oxidase in human neutrophils is modulated by the opacity-associated protein expression State of Neisseria gonorrhoeae.Infection and immunity · 2014Article
- The oxidative burst reaction in mammalian cells depends on gravity.Cell communication and signaling : CCS · 2013Article
- Protein Kinase C Epsilon Contributes to NADPH Oxidase Activation in a Pre-Eclampsia Lymphoblast Cell Model.Diseases (Basel, Switzerland) · 2013Article
- Mechanosensitive microRNAs-role in endothelial responses to shear stress and redox state.Free radical biology & medicine · 2013Review
- Midazolam induces cellular apoptosis in human cancer cells and inhibits tumor growth in xenograft mice.Molecules and cells · 2013Article
- Reactive oxygen species delay control of lymphocytic choriomeningitis virus.Cell death and differentiation · 2013Article
- NADPH oxidases in heart failure: poachers or gamekeepers?Antioxidants & redox signaling · 2013Review
- Regulation of alveolar macrophage p40phox: hierarchy of activating kinases and their inhibition by PGE2.Journal of leukocyte biology · 2012Article
- Regulation of NAD(P)H oxidases by AMPK in cardiovascular systems.Free radical biology & medicine · 2012Review
- Cell signaling through protein kinase C oxidation and activation.International journal of molecular sciences · 2012Review
- Phosphorylation of p47phox is required for receptor-mediated NADPH oxidase/NOX2 activation in Epstein-Barr virus-transformed human B lymphocytes.American journal of blood research · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The respiratory burst oxidase of phagocytes and B lymphocytes catalyzes the reduction of oxygen to O2- at the expense of NADPH. Dormant in resting cells, the oxidase is activated by exposing the cells to appropriate stimuli. During activation, p47phox, a cytosolic oxidase subunit, becomes extensively phosphorylated on a number of serines located between S303 and S379. To determine whether this phosphorylation is necessary for oxidase activation, we examined phorbol-elicited oxidase activity in EBV-transformed B lymphoblasts deficient in p47phox after transfection with plasmids expressing various S-->A mutants of p47phox. The mutant containing S-->A mutations involving all serines between S303 and S379 [S(303-379)A] was not phosphorylated, did not translocate to plasma membrane during activation and was almost devoid of function. As to individual serines, S379 was of special interest because (a) p47 phox S379 was phosphorylated in phorbol-activated lymphoblasts expressing wild-type p47phox, and (b) p47phox S379A failed to translocate to the membrane, and was as functionless as p47phox S(303-379)A; other single S-->A mutations had little effect on oxidase activity. These findings suggest that the phosphorylation of S379 may be important for oxidase activation in whole cells.
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