ArticleThe Journal of cell biology1995
Gamma-tubulin can both nucleate microtubule assembly and self-assemble into novel tubular structures in mammalian cells.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 107 citations in OpenAlex.
- Molecular determinants of intrinsic cellular stiffness in health and disease.Biophysical reviews · 2022Review
- γ-Tubulin in microtubule nucleation and beyond.Frontiers in cell and developmental biology · 2022Review
- The Application of Ultrasonic Vibration in Human Sperm Cryopreservation as a Novel Method for the Modification of Physicochemical Characteristics of Freezing Media.Scientific reports · 2019Article
- γ-Tubulin⁻γ-Tubulin Interactions as the Basis for the Formation of a Meshwork.International journal of molecular sciences · 2018Review
- Structural centrosome aberrations sensitize polarized epithelia to basal cell extrusion.Open biology · 2018Article
- Polo-like kinases (plks), a key regulator of cell cycle and new potential target for cancer therapy.Development & reproduction · 2014Article
- The cytoplasm of living cells behaves as a poroelastic material.Nature materials · 2013Article
- Signaling-dependent phosphorylation of mitotic centromere-associated kinesin regulates microtubule depolymerization and its centrosomal localization.The Journal of biological chemistry · 2012Article
- Analysis of centrosome localization of BRCA1 and its activity in suppressing centrosomal aster formation.Cell cycle (Georgetown, Tex.) · 2012Article
- Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis.The Journal of cell biology · 2005Article
- Cytoskeletal dynamics during mammalian gametegenesis and fertilization: Implications for human reproduction.Reproductive medicine and biology · 2005Review
- Human sperm centrosomal function during fertilization, a novel assessment for male sterility.Human cell · 2004Review
- Sperm membrane protein (hSMP-1) and RanBPM complex in the microtubule-organizing centre.Journal of molecular medicine (Berlin, Germany) · 2004Article
- Gamma-tubulin in basal land plants: characterization, localization, and implication in the evolution of acentriolar microtubule organizing centers.The Plant cell · 2004Article
- Chromosome instability in human lung cancers: possible underlying mechanisms and potential consequences in the pathogenesis.Oncogene · 2002Review
- Tetrahymena thermophila contains a conventional gamma-tubulin that is differentially required for the maintenance of different microtubule-organizing centers.The Journal of cell biology · 2002Article
- Characterization of Cep135, a novel coiled-coil centrosomal protein involved in microtubule organization in mammalian cells.The Journal of cell biology · 2002Article
- Human 76p: A new member of the gamma-tubulin-associated protein family.The Journal of cell biology · 1999Article
- The sudden recruitment of gamma-tubulin to the centrosome at the onset of mitosis and its dynamic exchange throughout the cell cycle, do not require microtubules.The Journal of cell biology · 1999Article
- When overexpressed, a novel centrosomal protein, RanBPM, causes ectopic microtubule nucleation similar to gamma-tubulin.The Journal of cell biology · 1998Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
alpha-, beta-, and gamma-tubulins are evolutionarily highly conserved members of the tubulin gene superfamily. While the abundant members, alpha- and beta-tubulins, constitute the building blocks of cellular microtubule polymers, gamma-tubulin is a low abundance protein which localized to the pericentriolar material and may play a role in microtubule assembly. To test whether gamma-tubulin mediates the nucleation of microtubule assembly in vivo, and co-assembles with alpha- and beta-tubulins into microtubules or self-assembles into macro-molecular structures, we experimentally elevated the expression of gamma-tubulin in the cell cytoplasm. In most cells, overexpression of gamma-tubulin causes a dramatic reorganization of the cellular microtubule network. Furthermore, we show that when overexpressed, gamma-tubulin causes ectopic nucleation of microtubules which are not associated with the centrosome. In a fraction of cells, gamma-tubulin self-assembles into novel tubular structures with a diameter of approximately 50 nm (named gamma-tubules). Furthermore, unlike microtubules, gamma-tubules are resistant to cold or drug induced depolymerization. These data provide evidence that gamma-tubulin can cause nucleation of microtubule assembly and can self-assemble into novel tubular structures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.