ArticleThe Journal of cell biology1995
A mutation in glyceraldehyde 3-phosphate dehydrogenase alters endocytosis in CHO cells.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 95 citations in OpenAlex.
- Glycolysis and glycolytic enzymes in acute myeloid leukemia: Warburg and beyond.Frontiers in pharmacology · 2026Review
- First In Silico Study of TwoInternational journal of molecular sciences · 2025Article
- AtCAP2 is crucial for lytic vacuole biogenesis during germination by positively regulating vacuolar protein trafficking.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- GAPDH binds Akt to facilitate cargo transport in the early secretory pathway.Experimental cell research · 2016Article
- Article
- Protein Recognition in Drug-Induced DNA Alkylation: When the Moonlight Protein GAPDH Meets S23906-1/DNA Minor Groove Adducts.International journal of molecular sciences · 2015Review
- Subcellular dynamics of multifunctional protein regulation: mechanisms of GAPDH intracellular translocation.Journal of cellular biochemistry · 2012Review
- Direct interaction between GluR2 and GAPDH regulates AMPAR-mediated excitotoxicity.Molecular brain · 2012Article
- Housekeeping gene selection advisory: glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and β-actin are targets of miR-644a.PloS one · 2012Article
- Testis-specific glyceraldehyde-3-phosphate dehydrogenase: origin and evolution.BMC evolutionary biology · 2011Article
- The diverse functions of GAPDH: views from different subcellular compartments.Cellular signalling · 2011Review
- Oxidatively modified glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Alzheimer's disease: many pathways to neurodegeneration.Journal of Alzheimer's disease : JAD · 2010Review
- A GAPDH mutant defective in Src-dependent tyrosine phosphorylation impedes Rab2-mediated events.Traffic (Copenhagen, Denmark) · 2007Article
- Regulation of oncogenic transcription factor hTAF(II)68-TEC activity by human glyceraldehyde-3-phosphate dehydrogenase (GAPDH).The Biochemical journal · 2007Article
- Glyceraldehyde 3-phosphate dehydrogenase is a SET-binding protein and regulates cyclin B-cdk1 activity.Oncogene · 2006Article
- Single-gene disorders: what role could moonlighting enzymes play?American journal of human genetics · 2005Review
- Interactions among p22, glyceraldehyde-3-phosphate dehydrogenase and microtubules.The Biochemical journal · 2004Article
- Pyruvate carboxylase is an essential protein in the assembly of yeast peroxisomal oligomeric alcohol oxidase.Molecular biology of the cell · 2003Article
- Tubulin is the endogenous inhibitor of the glyceraldehyde 3-phosphate dehydrogenase isoform that catalyzes membrane fusion: Implications for the coordinated regulation of glycolysis and membrane fusion.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Proliferative and nutritional dependent regulation of glyceraldehyde-3-phosphate dehydrogenase expression in the rat liver.Cell proliferation · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CHO cell mutant FD 1.3.25 exhibits both increased accumulation and altered distribution of endocytosed fluid phase tracers. Neither the rate of tracer internalization nor the kinetics of recycling from early endosomes was affected, but exocytosis from late endocytic compartments appeared to be decreased in the mutant. Endocytosed tracer moved more rapidly to the cell poles in FD1.3.25 than in wild type cells. An abundant 36-kD polypeptide was found associated with taxol-polymerized microtubules in preparations from wild type and mutant; in the former but not the latter this polypeptide could be dissociated by incubation of the microtubules in ATP or high salt. The 36-kD polypeptide co-electrophoresed in two dimensions with the monomer of the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Analysis of cDNA clones showed that the mutant is heterozygous for this enzyme, with approximately 25% of the GAPDH RNA containing a single nucleotide change resulting in substitution of Ser for Pro234, a residue that is conserved throughout evolution. Stable transfectants of wild type cells expressing the mutant monomer at approximately 15% of the total enzyme exhibited the various changes in endocytosis observed in FD1.3.25.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.