ArticleThe Biochemical journal1995
An upstream stimulatory factor (USF) binding motif is critical for rat preprotachykinin-A promoter activity in PC12 cells.
Article in The Biochemical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 27 citations in OpenAlex.
- Lithium chloride regulation of the substance P encoding preprotachykinin a, Tac1 gene in rat hippocampal primary cells.Journal of molecular neuroscience : MN · 2011Article
- Upstream stimulatory factors are mediators of Ca2+-responsive transcription in neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Upstream stimulatory factor activates the vasopressin promoter via multiple motifs, including a non-canonical E-box.The Biochemical journal · 2003Article
- E-box motifs within the human vasopressin gene promoter contribute to a major enhancer in small-cell lung cancer.The Biochemical journal · 1999Article
- Novel cell lines for the analysis of preprotachykinin A gene expression identify a repressor domain 3' of the major transcriptional start site.The Biochemical journal · 1999Article
- Molecular cloning, sequencing and functional study of the promoter region of the human alpha2C4-adrenergic receptor gene.The Biochemical journal · 1997Article
- Participation of altered upstream stimulatory factor in the induction of rat heme oxygenase-1 by cadmium.Nucleic acids research · 1996Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate the presence of a functional E box motif in the proximal rat preprotachykinin-A (rPPT) promoter. This element (spanning nucleotides -67 to -47) exhibits the sequence 5'-CACGTG-3' which is recognized and bound by the basic helix-loop-helix family of regulatory proteins. We also show that at least one of the factors bound to this rPPT promoter element in both HeLa and PC12 nuclear extract is the ubiquitously expressed transcription factor, the upstream stimulatory factor (USF). Mutation of this element by insertion of a 10 bp linker into the E box motif, in an rPPT promoter fragment spanning -865 to +92, destroys the ability of this promoter fragment to support reporter gene expression in a PC12 cell model of rPPT promoter activity. The data indicate that this rPPT E box element is likely to function as an important cis-regulatory domain in the rPPT promoter.
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