ArticleNucleic acids research1995
The effect of target site transcription on gene targeting in human cells in vitro.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Evaluating the Genomic Parameters Governing rAAV-Mediated Homologous Recombination.Molecular therapy : the journal of the American Society of Gene Therapy · 2021Article
- Review
- Silent IL2RG Gene Editing in Human Pluripotent Stem Cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2016Article
- A genome-wide map of adeno-associated virus-mediated human gene targeting.Nature structural & molecular biology · 2014Article
- Concerted nicking of donor and chromosomal acceptor DNA promotes homology-directed gene targeting in human cells.Nucleic acids research · 2012Article
- Chromosomal position effects on AAV-mediated gene targeting.Nucleic acids research · 2010Article
- Stimulation of homology-directed gene targeting at an endogenous human locus by a nicking endonuclease.Nucleic acids research · 2009Article
- Impact of non-homologous end-joining deficiency on random and targeted DNA integration: implications for gene targeting.Nucleic acids research · 2008Article
- RNA polymerase I transcription stimulates homologous recombination in Trypanosoma brucei.Molecular and biochemical parasitology · 2007Article
- Article
- Gene targeting in yeast is initiated by two independent strand invasions.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Efficient gene disruption in the koji-mold Aspergillus sojae using a novel variation of the positive-negative method.Molecular genetics and genomics : MGG · 2004Article
- Cis-acting regulatory sequences promote high-frequency gene conversion between repeated sequences in mammalian cells.Nucleic acids research · 2004Article
- Illegitimate DNA integration in mammalian cells.Gene therapy · 2003Review
- Human gene targeting by adeno-associated virus vectors is enhanced by DNA double-strand breaks.Molecular and cellular biology · 2003Article
- A chromosomal position effect on gene targeting in human cells.Nucleic acids research · 2002Article
- Hpr1 is preferentially required for transcription of either long or G+C-rich DNA sequences in Saccharomyces cerevisiae.Molecular and cellular biology · 2001Article
- Article
- A new hyperrecombination mutation identifies a novel yeast gene, THP1, connecting transcription elongation with mitotic recombination.Genetics · 2001Article
- A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae.The EMBO journal · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We evaluate the effect of target site transcription on gene targeting in cultured human fibrosarcoma cells. A number of cell lines that harbored a plasmid recombination substrate within their chromosomal DNA were created. Gene targeting frequency was then measured at these different loci in the presence and absence of an agent that stimulated target site transcription. We observed that gene targeting was significantly enhanced by RNA transcription. The magnitude of transcription-stimulated gene targeting varied from 3-fold to > 20-fold. No increase in gene targeting was observed, however, when transcription proceeded away from, rather than through, the recombination site. Transcription-stimulated gene targeting was also observed when single-stranded plasmid vectors complementary to either the coding or template strand were used as recombination substrates. Our results indicate that gene targeting, like other forms of DNA recombination, can be stimulated by target site transcription. The implications of our observations on current models of transcription-stimulated recombination are discussed.
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