ArticleNucleic acids research1995
The trinucleotide repeat sequence d(GTC)15 adopts a hairpin conformation.
Article in Nucleic acids research, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 45 citations in OpenAlex.
- Frustration Between Preferred States of Complementary Trinucleotide Repeat DNA Hairpins Anticorrelates with Expansion Disease Propensity.Journal of molecular biology · 2023Article
- Chromosomal directionality of DNA mismatch repair in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
- Comparative genomics and molecular dynamics of DNA repeats in eukaryotes.Microbiology and molecular biology reviews : MMBR · 2008Review
- Article
- Replication fork stalling at natural impediments.Microbiology and molecular biology reviews : MMBR · 2007Review
- Length-dependent energetics of (CTG)n and (CAG)n trinucleotide repeats.Nucleic acids research · 2005Article
- Transcription influences the types of deletion and expansion products in an orientation-dependent manner from GAC*GTC repeats.Nucleic acids research · 2004Article
- Modelling studies on neurodegenerative disease-causing triplet repeat sequences d(GGC/GCC)n and d(CAG/CTG)n.Journal of biosciences · 2001Article
- Multiple pathways of recombination induced by double-strand breaks in Saccharomyces cerevisiae.Microbiology and molecular biology reviews : MMBR · 1999Review
- Triplet repeats form secondary structures that escape DNA repair in yeast.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- NGG-triplet repeats form similar intrastrand structures: implications for the triplet expansion diseases.Nucleic acids research · 1998Article
- Cloned human FMR1 trinucleotide repeats exhibit a length- and orientation-dependent instability suggestive of in vivo lagging strand secondary structure.Nucleic acids research · 1998Article
- Trinucleotide repeats associated with human disease.Nucleic acids research · 1997Review
- Stability of a CTG/CAG trinucleotide repeat in yeast is dependent on its orientation in the genome.Molecular and cellular biology · 1997Article
- Single-stranded DNA-binding protein enhances the stability of CTG triplet repeats in Escherichia coli.Journal of bacteriology · 1996Article
- Stability of intrastrand hairpin structures formed by the CAG/CTG class of DNA triplet repeats associated with neurological diseases.Nucleic acids research · 1996Article
- The purine-rich trinucleotide repeat sequences d(CAG)15 and d(GAC)15 form hairpins.Nucleic acids research · 1995Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The structure of a single-stranded (ss) oligonucleotide containing (GTC)15 [ss(GTC)15] was examined. As a control, parallel studies were performed with ss(CTG)15, an oligonucleotide that forms a hairpin. Electrophoretic mobility, KMnO4 oxidation and P1 nuclease studies demonstrate that, similar to ss(CTG)15, ss(GTC)15 forms a hairpin containing base paired and/or stacked thymines in the stem. Electrophoretic mobility melting profiles performed in approximately 1 mM Na+ revealed that the melting temperature of ss(GTC)15 and ss(CTG)15 were 38 and 48 degrees C respectively. The loop regions of ss(GTC)15 and ss(CTG)15 were cleaved by single-strand-specific P1 nuclease at the T25-C29 and G26-C27 phosphodiester bonds respectively (where the loop apex of the DNAs is T28). Molecular dynamics simulations suggested that in ss(GTC)15 the loop was bent towards the major groove of the stem, apparently causing an increased exposure of the T25-C29 region to solvent. In ss(CTG)15 guanine--guanine stacking caused a separation of the G26 and C27 bases, resulting in exposure of the intervening phosphodiester to solvent. The results suggest that ss(GTC)15 and ss(CTG)15 form similar, but distinguishable, hairpin structures.
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