ArticleMolecular and cellular biology1995
A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer.
Article in Molecular and cellular biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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Who cites it
102 citing papers in PubMed, 179 citations in OpenAlex.
- Stretching muscle cells induces transcriptional and splicing transitions and changes in SR proteins.Communications biology · 2022Article
- Exonic splicing code and protein binding sites for calcium.Nucleic acids research · 2022Article
- Highly ABA-Induced 1 (HAI1)-Interacting protein HIN1 and drought acclimation-enhanced splicing efficiency at intron retention sites.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Comparative studies of Toxoplasma gondii transcriptomes: insights into stage conversion based on gene expression profiling and alternative splicing.Parasites & vectors · 2018Article
- POWERDRESS and HDA9 interact and promote histone H3 deacetylation at specific genomic sites in Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- RBFox1-mediated RNA splicing regulates cardiac hypertrophy and heart failure.The Journal of clinical investigation · 2016Article
- Binding site discovery from nucleic acid sequences by discriminative learning of hidden Markov models.Nucleic acids research · 2014Article
- Genome-Wide Analysis of Heat-Sensitive Alternative Splicing in Physcomitrella patens.Plant physiology · 2014Article
- Genome-wide analysis of light-regulated alternative splicing mediated by photoreceptors in Physcomitrella patens.Genome biology · 2014Article
- Global impact of RNA splicing on transcriptome remodeling in the heart.Journal of Zhejiang University. Science. B · 2012Review
- Predicting sequence and structural specificities of RNA binding regions recognized by splicing factor SRSF1.BMC genomics · 2011Article
- Compensatory signals associated with the activation of human GC 5' splice sites.Nucleic acids research · 2011Article
- Global analysis reveals SRp20- and SRp75-specific mRNPs in cycling and neural cells.Nature structural & molecular biology · 2010Article
- Transposable elements in disease-associated cryptic exons.Human genetics · 2010Article
- Delivery of bifunctional RNAs that target an intronic repressor and increase SMN levels in an animal model of spinal muscular atrophy.Human molecular genetics · 2009Article
- Splicing factor SFRS1 recognizes a functionally diverse landscape of RNA transcripts.Genome research · 2009Article
- Identification of nuclear and cytoplasmic mRNA targets for the shuttling protein SF2/ASF.PloS one · 2008Article
- Multisite and bidirectional exonic splicing enhancer in CD44 alternative exon v3.RNA (New York, N.Y.) · 2007Article
- Global control of aberrant splice-site activation by auxiliary splicing sequences: evidence for a gradient in exon and intron definition.Nucleic acids research · 2007Article
- Exonic splicing enhancers in fission yeast: functional conservation demonstrates an early evolutionary origin.Genes & development · 2005Article
42 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The cardiac troponin T pre-mRNA contains an exonic splicing enhancer that is required for inclusion of the alternative exon 5. Here we show that enhancer activity is exquisitely sensitive to changes in the sequence of a 9-nucleotide motif (GAGGAAGAA) even when its purine content is preserved. A series of mutations that increased or decreased the level of exon inclusion in vivo were used to correlate enhancer strength with RNA-protein interactions in vitro. Analyses involving UV cross-linking and immunoprecipitation indicated that only four (SRp30a, SRp40, SRp55, and SRp75) of six essential splicing factors known as SR proteins bind to the active enhancer RNA. Moreover, purified SRp40 and SRp55 activate splicing of exon 5 when added to a splicing-deficient S100 extract. Purified SRp30b did not stimulate splicing in S100 extracts, which is consistent with its failure to bind the enhancer RNA. In vitro competition of SR protein splicing activity and UV cross-linking demonstrated that the sequence determinants for SR protein binding were precisely coincident with the sequence determinants of enhancer strength. Thus, a subset of SR proteins interacts directly with the exonic enhancer to promote inclusion of a poorly defined alternative exon. Independent regulation of the levels of SR proteins may, therefore, contribute to the developmental regulation of exon inclusion.
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Registered trials
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