ArticleJournal of clinical microbiology1995
Comparison of three typing methods for clinical and environmental isolates of Aspergillus fumigatus.
Article in Journal of clinical microbiology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- Selection of Polymorphic Patterns Obtained by RAPD-PCR through Qualitative and Quantitative Analyses to DifferentiateJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- A review molecular typing methods for Aspergillus flavus isolates.Mycopathologia · 2011Review
- Discovery of a sexual cycle in the opportunistic fungal pathogen Aspergillus fumigatus.Nature · 2009Article
- RAPD typing of Aspergillus chevalieri, Aspergillus nidulans, Aspergillus tetrazonus (quadrilineatus) and their teleomorphs using 5'-d[AACGCGCAAC]-3' and 5'-d[CCCGTCAGCA]-3' primers.Molecular biology reports · 2008Article
- Characterization of a novel gene for strain typing reveals substructuring of Aspergillus fumigatus across North America.Eukaryotic cell · 2007Article
- Evaluation of performance of four genotypic methods for studying the genetic epidemiology of Aspergillus fumigatus isolates.Journal of clinical microbiology · 2002Article
- Phenotyping and genotyping of Sporothrix schenckii isolates according to geographic origin and clinical form of Sporotrichosis.Journal of clinical microbiology · 2002Article
- Epidemiology and Prevention of Invasive Aspergillosis.Current infectious disease reports · 2001Article
- Comparison of restriction fragment length polymorphism, microsatellite length polymorphism, and random amplification of polymorphic DNA analyses for fingerprinting Aspergillus fumigatus isolates.Journal of clinical microbiology · 2001Article
- Development of molecular methods for identification of Schizophyllum commune from clinical samples.Journal of clinical microbiology · 2001Article
- Genetic polymorphism of Aspergillus fumigatus in clinical samples from patients with invasive aspergillosis: investigation using multiple typing methods.Journal of clinical microbiology · 2001Article
- The ins and outs of DNA fingerprinting the infectious fungi.Clinical microbiology reviews · 2000Review
- Aspergillus fumigatus and aspergillosis.Clinical microbiology reviews · 1999Review
- Genetic multilocus studies of different strains of Cryptococcus neoformans: taxonomy and genetic structure.Journal of clinical microbiology · 1999Article
- The evolutionary biology and population genetics underlying fungal strain typing.Clinical microbiology reviews · 1999Review
- Microsatellite markers for typing Aspergillus fumigatus isolates.Journal of clinical microbiology · 1998Article
- Molecular typing of environmental and patient isolates of Aspergillus fumigatus from various hospital settings.Journal of clinical microbiology · 1998Article
- Molecular epidemiological study of Aspergillus fumigatus in a bone marrow transplantation unit by PCR amplification of ribosomal intergenic spacer sequences.Journal of clinical microbiology · 1998Article
- Genetic diversity among clinical and environmental isolates of Aspergillus fumigatus.Infection and immunity · 1997Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
To evaluate procedures used for epidemiologic analysis of outbreaks of aspergillosis, we analyzed a collection of 35 Aspergillus fumigatus isolates using three typing methods: isoenzyme analysis (IEA), random amplified polymorphic DNA (RAPD) analysis, and restriction endonuclease analysis (REA). Twenty-one isolates were from a single hospital, with four isolates coming from different patients. Three clinical isolates came from a different hospital, and 11 clinical or environmental isolates were derived from a culture collection. With IEA, the patterns of alkaline phosphatase, esterase, and catalase discriminated nine types. In contrast, 22 types were obtained with five different RAPD primers, and 21 types could be detected with three of these (R108, R151, and UBC90). Restriction endonuclease analysis of genomic DNA, digested with either XbaI, XhoI, or SalI, detected 3, 17, and 13 different REA types, respectively, and 22 types were identified by combining the data from the XhoI and SalI REAs. Twenty-eight types were obtainable with a combination of REA, IEA, and RAPD patterns. Overall, the results pointed to substantial genetic variation among the isolates. Though two isolates had markedly distinct genotypes, their morphologic features and exoantigens were consistent with their being A. fumigatus. The analysis will help in planning epidemiologic studies of aspergillosis.
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