ArticleThe Plant cell1995
A nodule-specific gene encoding a subtilisin-like protease is expressed in early stages of actinorhizal nodule development.
Article in The Plant cell, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 171 citations in OpenAlex.
- Natural Variation of NAR5 Determines Nitrogenase Activity and the Yield in Soybean.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Legume NCRs and nodule-specific defensins of actinorhizal plants-Do they share a common origin?PloS one · 2022Article
- Anthoceros genomes illuminate the origin of land plants and the unique biology of hornworts.Nature plants · 2020Article
- Article
- Overexpression of aFrontiers in plant science · 2018Article
- Comparative Analysis of the Nodule Transcriptomes ofFrontiers in plant science · 2018Article
- Gene expression atlas of pigeonpea and its application to gain insights into genes associated with pollen fertility implicated in seed formation.Journal of experimental botany · 2017Article
- zmsbt1 and zmsbt2, two new subtilisin-like serine proteases genes expressed in early maize kernel development.Planta · 2017Article
- Genome-wide and molecular evolution analysis of the subtilase gene family in Vitis vinifera.BMC genomics · 2014Article
- A brief history of Frankia and actinorhizal plants meetings.Journal of biosciences · 2013Article
- Biological nitrogen fixation in non-legume plants.Annals of botany · 2013Review
- Article
- Transcriptomics of actinorhizal symbioses reveals homologs of the whole common symbiotic signaling cascade.Plant physiology · 2011Article
- Plasmodesmata distribution and sugar partitioning in nitrogen-fixing root nodules of Datisca glomerata.Planta · 2011Article
- Functional analysis of the cucumisin propeptide as a potent inhibitor of its mature enzyme.The Journal of biological chemistry · 2010Article
- IiSDD1, a gene responsive to autopolyploidy and environmental factors in Isatis indigotica.Molecular biology reports · 2010Article
- Crystallization and preliminary X-ray analysis of cryptolepain, a novel glycosylated serine protease from Cryptolepis buchanani.Acta crystallographica. Section F, Structural biology and crystallization communications · 2007Article
- Analysis of xylem sap proteins from Brassica napus.BMC plant biology · 2005Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To identify genes specifically expressed during early stages of actinorhizal nodule development, a cDNA library made from poly(A) RNA from root nodules of Alnus glutinosa was screened differentially with nodule and root cDNA, respectively. Seven nodule-enhanced and four nodule-specific cDNA clones were isolated. By using in situ hybridization, two of the nodule-specific cDNAs were shown to be expressed at the highest levels in infected cells before the onset of nitrogen fixation; one of them, ag12 (A. glutinosa), was examined in detail. Sequencing showed that ag12 codes for a serine protease of the subtilisin (EC 3.4.21.14) family. Subtilisins previously appeared to be limited to microorganisms. However, subtilisin-like serine proteases have recently been found in archaebacteria, fungi, and yeasts as well as in mammals; a plant subtilisin has also been sequenced. In yeast and mammals, subtilases are responsible for processing peptide hormones. A homolog of ag12, ara12, was identified in Arabidopsis; it was expressed in all organs, and its expression levels were highest during silique development. Hence, our study shows that subtilases are also involved in both symbiotic and nonsymbiotic processes in plant development.
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