ArticleThe Biochemical journal1995
Structural analysis of inositol phospholipids from Trypanosoma cruzi epimastigote forms.
Article in The Biochemical journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Mass Spectrometry-Based Metabolomics Revealed Effects of Metronidazole onPharmaceuticals (Basel, Switzerland) · 2023Article
- Biological and Molecular Effects ofFrontiers in cellular and infection microbiology · 2021Article
- Comparative sphingolipidomics of disease-causing trypanosomatids reveal unique lifecycle- and taxonomy-specific lipid chemistries.Scientific reports · 2017Article
- Article
- Ether-type moieties in the lipid part of glycoinositolphospholipids of Acanthamoeba rhysodes.Lipids · 2014Article
- Lipid synthesis in protozoan parasites: a comparison between kinetoplastids and apicomplexans.Progress in lipid research · 2013Review
- The Sphingolipid Biosynthetic Pathway Is a Potential Target for Chemotherapy against Chagas Disease.Enzyme research · 2011Article
- Lipid metabolism in Trypanosoma brucei.Molecular and biochemical parasitology · 2010Review
- Lipidomic analysis of bloodstream and procyclic form Trypanosoma brucei.Parasitology · 2010Review
- Cell-free synthesis and functional characterization of sphingolipid synthases from parasitic trypanosomatid protozoa.The Journal of biological chemistry · 2010Article
- The essential neutral sphingomyelinase is involved in the trafficking of the variant surface glycoprotein in the bloodstream form of Trypanosoma brucei.Molecular microbiology · 2010Article
- Metabolic response to iron deficiency in Saccharomyces cerevisiae.The Journal of biological chemistry · 2010Article
- Sphingolipids in parasitic protozoa.Advances in experimental medicine and biology · 2010Review
- Developmentally regulated sphingolipid synthesis in African trypanosomes.Molecular microbiology · 2008Article
- Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi.Molecular and biochemical parasitology · 2008Article
- De novo sphingolipid synthesis is essential for viability, but not for transport of glycosylphosphatidylinositol-anchored proteins, in African trypanosomes.Eukaryotic cell · 2007Article
- GPI-anchored proteins and free GPI glycolipids of procyclic form Trypanosoma brucei are nonessential for growth, are required for colonization of the tsetse fly, and are not the only components of the surface coat.Molecular biology of the cell · 2006Article
- A mammalian fatty acid hydroxylase responsible for the formation of alpha-hydroxylated galactosylceramide in myelin.The Biochemical journal · 2005Article
- Characterization of the inositol phosphorylceramide synthase activity from Trypanosoma cruzi.The Biochemical journal · 2005Article
- Formation and remodeling of inositolphosphoceramide during differentiation of Trypanosoma cruzi from trypomastigote to amastigote.Eukaryotic cell · 2003Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inositol phospholipids (IPL) from epimastigote forms of Trypanosoma cruzi have been investigated by metabolic labelling with [3H]palmitic acid and by GLC-MS analysis of the lipids obtained from non-labelled parasites. The IPL fraction was separated into phosphatidylinositol (PI) and inositol-phosphoceramide subfractions, the latter accounting for 80-85% of the total IPL. The neutral lipids released from the IPLs by PI-specific phospholipase C (PI-PLC) from Bacillus thuringiensis were analysed by silica-gel and reverse-phase TLC for the radioactive lipids and by GLC-MS for the non-radioactive samples. Ceramides containing dihydrosphingosine and sphingosine with C16:0 and C18:0 fatty acids were identified. The main component in the [3H]palmitic acid-labelled ceramides was palmitoyldihydrospingosine, while in the non-labelled sample the ceramides contained mainly sphingosine. This could reflect partial uptake of phospholipid from the medium. The PI contain both alkylacyl- and diacyl-glycerol lipids, with the ether lipid being more abundant. The latter was identified as 1-O-hexadecylglycerol esterified by C18:2 and C18:1 fatty acids. Interestingly, the same lipid had been identified in the anchor of the 1G7 glycoprotein of T. cruzi metacyclic forms.
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