ArticleThe Journal of cell biology1995
Transfected connexin45 alters gap junction permeability in cells expressing endogenous connexin43.
Article in The Journal of cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 172 citations in OpenAlex.
- Cx45 regulation by kinases and impact of expression in heart failure.Journal of molecular and cellular cardiology · 2025Article
- Role of Cx43 on the Bone Cell Generation, Function, and Survival.Bioelectricity · 2023Article
- Intramolecular signaling in a cardiac connexin: Role of cytoplasmic domain dimerization.Journal of molecular and cellular cardiology · 2017Article
- Connexin subtype expression during oral carcinogenesis: A pilot study in patients with oral squamous cell carcinoma.Molecular and clinical oncology · 2016Article
- Connexins in the skeleton.Seminars in cell & developmental biology · 2016Review
- Junctional abnormalities in human airway epithelial cells expressing F508del CFTR.American journal of physiology. Lung cellular and molecular physiology · 2015Article
- Up-regulation of BMP2/4 signaling increases both osteoblast-specific marker expression and bone marrow adipogenesis in Gja1Jrt/+ stromal cell cultures.Molecular biology of the cell · 2015Article
- Characterization of the connexin45 carboxyl-terminal domain structure and interactions with molecular partners.Biophysical journal · 2014Article
- Regulation of cellular communication by signaling microdomains in the blood vessel wall.Pharmacological reviews · 2014Review
- Article
- Review
- Organ explant culture of neonatal rat ventricles: a new model to study gene and cell therapy.PloS one · 2013Article
- Gap junctions and hemichannels in signal transmission, function and development of bone.Biochimica et biophysica acta · 2012Review
- Cytoplasmic amino acids within the membrane interface region influence connexin oligomerization.The Journal of membrane biology · 2012Article
- Different domains are critical for oligomerization compatibility of different connexins.The Biochemical journal · 2011Article
- Regulation of cellular function by connexin hemichannels.International journal of biochemistry and molecular biology · 2011Article
- Characterization of hTERT-immortalized osteoblast cell lines generated from wild-type and connexin43-null mouse calvaria.American journal of physiology. Cell physiology · 2010Article
- Connexin43 potentiates osteoblast responsiveness to fibroblast growth factor 2 via a protein kinase C-delta/Runx2-dependent mechanism.Molecular biology of the cell · 2009Article
- ERp29 restricts Connexin43 oligomerization in the endoplasmic reticulum.Molecular biology of the cell · 2009Article
- Differential expression of connexins during histogenesis of the chick retina.Developmental neurobiology · 2008Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Many cells express multiple connexins, the gap junction proteins that interconnect the cytosol of adjacent cells. Connexin43 (Cx43) channels allow intercellular transfer of Lucifer Yellow (LY, MW = 443 D), while connexin45 (Cx45) channels do not. We transfected full-length or truncated chicken Cx45 into a rat osteosarcoma cell line ROS-17/2.8, which expresses endogenous Cx43. Both forms of Cx45 were expressed at high levels and colocalized with Cx43 at plasma membrane junctions. Cells transfected with full-length Cx45 (ROS/Cx45) and cells transfected with Cx45 missing the 37 carboxyl-terminal amino acids (ROS/Cx45tr) showed 30-60% of the gap junctional conductance exhibited by ROS cells. Intercellular transfer of three negatively charged fluorescent reporter molecules was examined. In ROS cells, microinjected LY was transferred to an average of 11.2 cells/injected cell, while dye transfer between ROS/Cx45 cells was reduced to 3.9 transfer between ROS/Cx45 cells was reduced to 3.9 cells. In contrast, ROS/Cx45tr cells transferred LY to > 20 cells. Transfer of calcein (MW = 623 D) was also reduced by approximately 50% in ROS/Cx45 cells, but passage of hydroxycoumarin carboxylic acid (HCCA; MW = 206 D) was only reduced by 35% as compared to ROS cells. Thus, introduction of Cx45 altered intercellular coupling between cells expressing Cx43, most likely the result of direct interaction between Cx43 and Cx45. Transfection of Cx45tr and Cx45 had different effects in ROS cells, consistent with a role of the carboxyl-terminal domain of Cx45 in determining gap junction permeability or interactions between connexins. These data suggest that coexpression of multiple connexins may enable cells to achieve forms of intercellular communication that cannot be attained by expression of a single connexin.
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