ArticleImmunogenetics1995
The recombination activation gene 1 (RAG1) of rainbow trout (Oncorhynchus mykiss): cloning, expression, and phylogenetic analysis.
Article in Immunogenetics, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 79 citations in OpenAlex.
- Article
- Review
- Long-Lived Plasma Cells Secrete High-Affinity Antibodies Responding to a T-Dependent Immunization in a Teleost Fish.Frontiers in immunology · 2019Article
- Novel Insights on the Regulation of B Cell Functionality by Members of the Tumor Necrosis Factor Superfamily in Jawed Fish.Frontiers in immunology · 2018Review
- Recombination-activating gene 1 and 2 (RAG1 and RAG2) in flounder (Paralichthys olivaceus).Journal of biosciences · 2014Article
- RAG-1 and IgM Genes, Markers for Early Development of the Immune System in Olive Flounder, Paralichthys olivaceus.Development & reproduction · 2014Article
- The astonishing diversity of Ig classes and B cell repertoires in teleost fish.Frontiers in immunology · 2013Article
- Lymphoid tissue ontogeny in the mummichog, Fundulus heteroclitus.Anatomical record (Hoboken, N.J. : 2007) · 2008Article
- Adapting to a changing world: RAG genomics and evolution.Human genomics · 2005Review
- The RAG proteins in V(D)J recombination: more than just a nuclease.Nucleic acids research · 2001Review
- Late changes in spliceosomal introns define clades in vertebrate evolution.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Roles of the "dispensable" portions of RAG-1 and RAG-2 in V(D)J recombination.Molecular and cellular biology · 1999Article
- Distinct factors regulate the murine RAG-2 promoter in B- and T-cell lines.Molecular and cellular biology · 1999Article
- Primordial emergence of the recombination activating gene 1 (RAG1): sequence of the complete shark gene indicates homology to microbial integrases.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- The recombination activating gene 2 (RAG2) of the rainbow trout Oncorhynchus mykiss.Immunogenetics · 1996Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The characterization of genes involved in the generation of the immune repertoire is an active area of research in lower vertebrate taxa. The recombination activating genes (RAG) have been shown to be essential for V (D) J recombination of T-cell antigen receptor (TCR) and immunoglobulin (Ig) genes, leading to the generation of the primary repertoire. As RAG1 is critical to the differentiation of pre-B and -T cells, its expression within an associated primary lymphoid organ can serve as a developmental marker. To examine the ontogeny of lymphocytes in Oncorhynchus mykiss, we cloned RAG1 from trout and examined its tissue- and lymphocyte-specific expression. The polymerase chain reaction, coupled with degenerate oligonucleotide primers, was used to amplify a homologous probe [(633 base pairs) (bp)] from rainbow trout genomic DNA, which in turn was used to isolate a lambda genomic clone. Sequence analysis of this genomic clone confirmed the RAG1 nature of this gene (3888 bp) and revealed an internal intron of 666 bp. When compared with other previously reported RAG1 sequences, the predicted amino acid translation (1073 aa) displayed a minimum of 78% similarity for the complete sequence and 89% similarity in the conserved region (aa 417-1042). Using northern blot analysis, we found the expression of RAG1 to be limited to surface Ig-n lymphocytes within the thymus. This data forms the basis for a proposal that the thymus of teleost species plays an essential developmental role in lymphopoiesis and thus can be regarded as a primary lymphoid organ.
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