ArticleThe EMBO journal1995
In vivo evidence for involvement of a 58 kDa component of nuclear pore-targeting complex in nuclear protein import.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 93 papers.
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Who cites it
93 citing papers in PubMed, 284 citations in OpenAlex.
- Reconstitution of nuclear envelope subdomain formation on mitotic chromosomes in semi-intact cells.Cell structure and function · 2024Article
- Analysis of the effects of importin α1 on the nuclear translocation of IL-1α in HeLa cells.Scientific reports · 2024Article
- Gene alterations in the nuclear transport receptor superfamily: A study of head and neck cancer.PloS one · 2024Article
- KPNA2 suppresses porcine epidemic diarrhea virus replication by targeting and degrading virus envelope protein through selective autophagy.Journal of virology · 2023Article
- Analysis of Ser/Thr Kinase HASPIN-Interacting Proteins in the Spermatids.International journal of molecular sciences · 2022Article
- Methods to separate nuclear soluble fractions reflecting localizations in living cells.iScience · 2021Article
- Distinct mutations in importin-β family nucleocytoplasmic transport receptors transportin-SR and importin-13 affect specific cargo binding.Scientific reports · 2021Article
- Article
- Increased Nuclear Transporter KPNA2 Contributes to Tumor Immune Evasion by Enhancing PD-L1 Expression in PDAC.Journal of immunology research · 2021Article
- Cellular Importin-α3 Expression Dynamics in the Lung Regulate Antiviral Response Pathways against Influenza A Virus Infection.Cell reports · 2020Article
- Thioredoxin-related transmembrane protein 2 (TMX2) regulates the Ran protein gradient and importin-β-dependent nuclear cargo transport.Scientific reports · 2019Article
- Review
- Structural basis for the nuclear import and export functions of the biportin Pdr6/Kap122.The Journal of cell biology · 2019Article
- Nuclear transport adapts to varying heat stress in a multistep mechanism.The Journal of cell biology · 2018Article
- Importin α: functions as a nuclear transport factor and beyond.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2018Review
- Aptamers that bind specifically to human KPNA2 (importin-α1) and efficiently interfere with nuclear transport.Journal of biochemistry · 2016Article
- Cell surface localization of importin α1/KPNA2 affects cancer cell proliferation by regulating FGF1 signalling.Scientific reports · 2016Article
- Calcium-Dependent Protein Kinase in Ginger Binds with Importin-α through Its Junction Domain for Nuclear Localization, and Further Interacts with NAC Transcription Factor.Frontiers in plant science · 2016Article
- Retinoblastoma-binding Protein 4-regulated Classical Nuclear Transport Is Involved in Cellular Senescence.The Journal of biological chemistry · 2015Article
- Purification of nuclear localization signal-containing proteins and its application to investigation of the mechanisms of the cell division cycle.Small GTPases · 2015Article
33 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We recently showed that a nuclear location signal (NLS)-containing karyophile forms a stable complex with cytoplasmic components for nuclear pore-targeting The complex, termed nuclear pore-targeting complex (PTAC), contained two essential proteins of 54 and 90 kDa, respectively, as estimated by electrophoresis. In this study, we found that the 54 kDa component of PTAC is the mouse homologue of Xenopus importin (m-importin). Cytoplasmic injection of the antibodies raised against recombinant m-importin showed an inhibitory effect on nuclear import of a karyophile in living mammalian cells. A portion of cytoplasmically injected antibodies migrated rapidly into the nucleus, indicating dynamic movement of this protein across the nuclear envelope. Moreover, the injected antibodies co-precipitated the karyophile, in an NLS-dependent manner, with endogenous m-importin in the cytoplasm. These results provide in vivo evidence that m-importin is involved in nuclear protein import through association with a NLS in the cytoplasm before nuclear pore binding.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.