ArticleThe EMBO journal1995
Membrane insertion and assembly of ductin: a polytopic channel with dual orientations.
Article in The EMBO journal, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 95 citations in OpenAlex.
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- Reversible binding of divalent cations toFrontiers in molecular biosciences · 2023Review
- A Meta-Analysis of Bioelectric Data in Cancer, Embryogenesis, and Regeneration.Bioelectricity · 2021Article
- Resolving the topological enigma in CaThe Journal of biological chemistry · 2019Review
- The lipid-dependent structure and function of LacY can be recapitulated and analyzed in phospholipid-containing detergent micelles.Scientific reports · 2019Article
- Epitope mapping of anti-PGRMC1 antibodies reveals the non-conventional membrane topology of PGRMC1 on the cell surface.Scientific reports · 2019Article
- Inverting the Topology of a Transmembrane Protein by Regulating the Translocation of the First Transmembrane Helix.Molecular cell · 2016Article
- Relating proton pumps with gap junctions: colocalization of ductin, the channel-forming subunit c of V-ATPase, with subunit a and with innexins 2 and 3 during Drosophila oogenesis.BMC developmental biology · 2016Article
- Control of lysosomal biogenesis and Notch-dependent tissue patterning by components of the TFEB-V-ATPase axis in Drosophila melanogaster.Autophagy · 2016Article
- Phospholipid Scramblase 1 Modulates FcR-Mediated Phagocytosis in Differentiated Macrophages.PloS one · 2016Article
- Article
- Reorientation of the first signal-anchor sequence during potassium channel biogenesis at the Sec61 complex.The Biochemical journal · 2013Article
- Estimating the rotation rate in the vacuolar proton-ATPase in native yeast vacuolar membranes.European biophysics journal : EBJ · 2013Article
- Molecular genetic and biochemical approaches for defining lipid-dependent membrane protein folding.Biochimica et biophysica acta · 2012Review
- A cytotoxic type III secretion effector of Vibrio parahaemolyticus targets vacuolar H+-ATPase subunit c and ruptures host cell lysosomes.PLoS pathogens · 2012Article
- Contribution of topology determinants of a viral movement protein to its membrane association, intracellular traffic, and viral cell-to-cell movement.Journal of virology · 2011Article
- Lipids and topological rules of membrane protein assembly: balance between long and short range lipid-protein interactions.The Journal of biological chemistry · 2011Article
- An emerging consensus for the structure of EmrE.Acta crystallographica. Section D, Biological crystallography · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Ductin is a highly conserved and polytopic transmembrane protein which is the subunit c component of the vacuolar H(+)-ATPase (V-ATPase) and a component of a connexon channel of gap junctions. Previous studies have suggested that ductin in the V-ATPase has the opposite orientation of ductin in a connexon. Using an in vitro translation system coupled to microsomes derived from the endoplasmic reticulum, we show that ductin is co-translationally inserted into the membrane bilayer, suggesting a dependency on the signal recognition particle for synthesis. By attaching a C-terminal polypeptide derived from beta-lactamase and by using cysteine replacement coupled to chemical labelling, we show that ductin is inserted into the microsomal membrane in both orientations in similar proportions. In contrast, squid rhodopsin appears to be inserted in a single orientation. Changing conserved charged residues at the N-terminus of ductin does not affect the ratio of the two orientations. Once in the microsomal membrane, ductin assembles into an oligomeric complex which contains a pore accessible to a water-soluble probe, reminiscent of the ductin complex found in the V-ATPase and a connexon.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.