Evidence map›Paper›PMID 7641067›Full record

ReviewHistochemistry and cell biology1995

Immunocytochemistry and in situ hybridization in the electron microscope: combined application in the study of virus-infected cells.

K Bienz, D Egger

Abstract readReview
PubMed Publisher
In one paragraph

Review in Histochemistry and cell biology, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
2.3field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 21 citations in OpenAlex.

  1. Wrapping things up about virus RNA replication.Traffic (Copenhagen, Denmark) · 2005
    Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. In situ hybridization: methods and applications.Journal of clinical laboratory analysis · 1997
    Review
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

K BienzInstitute for Medical Microbiology, University of Basel, Switzerland.
D Egger
University of Basel · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The present review evaluates methods for electron microscopic immunocytochemistry and in situ hybridization, using post-embedding techniques and colloidal gold as a label. Special emphasis is given to double labeling immunocytochemistry and double in situ hybridization and to their combined application on the same specimen. Brief guidelines are presented for fixation, embedding media, the use of polyclonal and monoclonal antibodies and nucleic acid probes. Conditions for labeling and binding of antibody and nucleic acid probes to the target and protocols for direct and indirect immunodetection are discussed. Combinations of direct and indirect immunodetections in multiple labeling experiments are summarized.

Indexed as

AntibodiesCarrier ProteinsCells, CulturedImmunohistochemistryIn Situ HybridizationMicroscopy, ElectronNucleic Acid ProbesPoliovirusRNA, ViralTissue EmbeddingTissue FixationViral Nonstructural ProteinsViral Proteins2C protein, viralAntibodiesCarrier ProteinsNucleic Acid ProbesRNA, ViralViral Nonstructural ProteinsViral Proteins

Identifiers

PMID7641067
OpenAlexW2094341721

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.