ArticleProceedings of the National Academy of Sciences of the United States of America1995
In vitro generation of hematopoietic stem cells from an embryonic stem cell line.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 145 citations in OpenAlex.
- Optimization of seeding density of OP9 cells to improve hematopoietic differentiation efficiency.BMC molecular and cell biology · 2024Article
- Effects of Simulated Microgravity on Wild Type and Marfan hiPSCs-Derived Embryoid Bodies.Cellular and molecular bioengineering · 2021Article
- Generation and manipulation of human iPSC-derived platelets.Cellular and molecular life sciences : CMLS · 2021Review
- Microgravity Reduces the Differentiation and Regenerative Potential of Embryonic Stem Cells.Stem cells and development · 2015Article
- Hematopoietic cell differentiation from embryonic and induced pluripotent stem cells.Stem cell research & therapy · 2013Review
- Induction of hematopoietic differentiation of mouse embryonic stem cells by an AGM-derived stromal cell line is not further enhanced by overexpression of HOXB4.Stem cells and development · 2010Article
- Autologous blood cell therapies from pluripotent stem cells.Blood reviews · 2010Review
- Surface antigen phenotypes of hematopoietic stem cells from embryos and murine embryonic stem cells.Blood · 2009Article
- Role of bioinspired polymers in determination of pluripotent stem cell fate.Regenerative medicine · 2009Review
- HOXB4 but not BMP4 confers self-renewal properties to ES-derived hematopoietic progenitor cells.Transplantation · 2008Article
- Embryonic stem cells make human T cells.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Lectin binding profiles of SSEA-4 enriched, pluripotent human embryonic stem cell surfaces.BMC developmental biology · 2005Article
- Embryonic stem cells as an alternate marrow donor source: engraftment without graft-versus-host disease.The Journal of experimental medicine · 2004Article
- Isolation and therapeutic potential of human haemopoietic stem cells.Cytotechnology · 2003Article
- Hematopoietic colony-forming cells derived from human embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Development of hematopoietic cells from embryonic stem cells.International journal of hematology · 2001Review
- Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- In vitro-generated neural precursors participate in mammalian brain development.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Hemato-lymphoid in vivo reconstitution potential of subpopulations derived from in vitro differentiated embryonic stem cells.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Stem cells of the respiratory epithelium and their in vitro cultivation.In vitro cellular & developmental biology. Animal · 1997Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hematopoietic stem cells (HSC) are unique in that they give rise both to new stem cells (self-renewal) and to all blood cell types. The cellular and molecular events responsible for the formation of HSC remain unknown mainly because no system exists to study it. Embryonic stem (ES) cells were induced to differentiate by coculture with the stromal cell line RP010 and the combination of interleukin (IL) 3, IL-6, and F (cell-free supernatants from cultures of the FLS4.1 fetal liver stromal cell line). Cell cytometry analysis of the mononuclear cells produced in the cultures was consistent with the presence of PgP-1+ Lin- early hematopoietic (B-220- Mac-1- JORO 75- TER 119-) cells and of fewer B-220+ IgM- B-cell progenitors and JORO 75+ T-lymphocyte progenitors. The cell-sorter-purified PgP-1+ Lin- cells produced by induced ES cells could repopulate the lymphoid, myeloid, and erythroid lineages of irradiated mice. The ES-derived PgP-1+ Lin- cells must possess extensive self-renewal potential, as they were able to produce hematopoietic repopulation of secondary mice recipients. Indeed, marrow cells from irradiated mice reconstituted (15-18 weeks before) with PgP-1+ Lin- cell-sorter-purified cells generated by induced ES cells repopulated the lymphoid, myeloid, and erythroid lineages of secondary mouse recipients assessed 16-20 weeks after their transfer into irradiated secondary mice. The results show that the culture conditions described here support differentiation of ES cells into hematopoietic cells with functional properties of HSC. It should now be possible to unravel the molecular events leading to the formation of HSC.
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