ArticleProceedings of the National Academy of Sciences of the United States of America1995
Temporal and molecular characteristics of mutations induced by ethylnitrosourea in germ cells isolated from seminiferous tubules and in spermatozoa of lacZ transgenic mice.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 90 citations in OpenAlex.
- The functional mutational landscape of theiScience · 2023Article
- Effect of sampling time on somatic and germ cell mutations induced by acrylamide in gpt delta mice.Genes and environment : the official journal of the Japanese Environmental Mutagen Society · 2021Article
- Chemically induced mutations in a MutaMouse reporter gene inform mechanisms underlying human cancer mutational signatures.Communications biology · 2020Article
- The development and prevalidation of an in vitro mutagenicity assay based on MutaMouse primary hepatocytes, Part II: Assay performance for the identification of mutagenic chemicals.Environmental and molecular mutagenesis · 2019Article
- Next generation sequencing of benzo(a)pyrene-induced lacZ mutants identifies a germ cell-specific mutation spectrum.Scientific reports · 2016Article
- Characterizing Benzo[a]pyrene-induced lacZ mutation spectrum in transgenic mice using next-generation sequencing.BMC genomics · 2015Article
- Sublinear response in lacZ mutant frequency of Muta™ Mouse spermatogonial stem cells after low dose subchronic exposure to N-ethyl-N-nitrosourea.Environmental and molecular mutagenesis · 2015Article
- Transgenic rodent assay for quantifying male germ cell mutant frequency.Journal of visualized experiments : JoVE · 2014Article
- Isolated spermatozoa as indicators of mutations transmitted to progeny.Mutation research · 2010Article
- Unidirectional startle responses and disrupted left-right co-ordination of motor behaviors in robo3 mutant zebrafish.Genes, brain, and behavior · 2009Article
- Assessing human germ-cell mutagenesis in the Postgenome Era: a celebration of the legacy of William Lawson (Bill) Russell.Environmental and molecular mutagenesis · 2007Article
- Article
- Mutagenicity testing with transgenic mice. Part II: Comparison with the mouse spot test.Journal of carcinogenesis · 2005Article
- Mutagenicity testing with transgenic mice. Part I: Comparison with the mouse bone marrow micronucleus test.Journal of carcinogenesis · 2005Article
- Databases and software for the analysis of mutations in the human p53 gene, human hprt gene and both the lacI and lacZ gene in transgenic rodents.Nucleic acids research · 1998Article
- Databases and software for the analysis of mutations in the human p53 gene, the human hprt gene and both the lacI and lacZ gene in transgenic rodents.Nucleic acids research · 1997Article
- Databases and software for the analysis of mutations in the human p53 gene, the human hprt gene and the lacZ gene in transgenic rodents.Nucleic acids research · 1996Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The lacZ transgenic mouse (Muta mouse) model was used to examine the timing of ethylnitrosourea (ENU)-induced mutations in germ cells. The spectrum of mutations was also determined. Animals received five daily treatments with ENU at 50 mg/kg and were sampled at times up to 55 days after treatment. In mixed germ-cell populations isolated from seminiferous tubules, there was little increase in the mutant frequency 5 days after treatment; subsequently, there was a continuous increase until the maximum (17.5-fold above background) was reached by approximately 35 days. In the spermatozoa, an increase in mutant frequency was not seen until 20 days after treatment, with the maximum (4.3-fold above background) being achieved no sooner than approximately 35 days. Based on the timing of sampling, these data demonstrate the detection of both spermatogonial and postspermatogonial, mutations. The most prominent feature of the ENU-induced base-pair mutations in testicular germ cells sampled 55 days after treatment is that 70% are induced in A.T base pairs, compared to only 16% in spontaneous mutations. These findings are consistent with comparable data from ENU studies using assays for inherited germ-cell mutations in mice. This study has demonstrated the utility and potential of the transgenic mouse lacZ model (Muta mouse) for the detection and study of germ-cell mutations and provides guidance in the selection of simplified treatment and sampling protocols.
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