ArticleProceedings of the National Academy of Sciences of the United States of America1995
Sp1 functions in a chromatin-dependent manner to augment human alpha-globin promoter activity.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 30 citations in OpenAlex.
- DNA G-quadruplex structures act as functional elements in α- and β-globin enhancers.Genome biology · 2025Article
- Long-term exposure of K562 cells to benzene metabolites inhibited erythroid differentiation and elevated methylation in erythroid specific genes.Toxicology research · 2016Article
- Annotation of cis-regulatory elements by identification, subclassification, and functional assessment of multispecies conserved sequences.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Structural and functional cross-talk between a distant enhancer and the epsilon-globin gene promoter shows interdependence of the two elements in chromatin.Molecular and cellular biology · 1999Article
- Characterization of novel parent-specific epigenetic modifications upstream of the imprinted mouse H19 gene.Molecular and cellular biology · 1998Article
- Xenopus TFIIIA gene transcription is dependent on cis-element positioning and chromatin structure.Molecular and cellular biology · 1998Article
- CpG islands from the alpha-globin gene cluster increase gene expression in an integration-dependent manner.Molecular and cellular biology · 1997Article
- Proximal promoter elements of the human zeta-globin gene confer embryonic-specific expression on a linked reporter gene in transgenic mice.Nucleic acids research · 1996Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The 5' flanking region of the human alpha-globin gene is highly G + C rich and contains multiple copies of the consensus sequence for the Sp1 binding site. We investigated the role of this G + C-rich region in augmenting alpha-globin promoter activity in the presence of the far-upstream alpha-globin enhancer, HS-40. We show that in transiently transfected erythroid cells, deletion of the alpha-globin G + C-rich 5' flanking region has no effect on alpha-globin promoter activity. However, upon stable integration into chromatin, deletion of this region causes a nearly 90% decrease in promoter activity compared with expression from an alpha-globin promoter retaining this region. These results suggest that the alpha-globin G + C-rich 5' flanking region augments alpha-globin promoter activity in a chromatin-dependent manner. We further show that this G + C-rich region is required for the activation of alpha-globin gene expression during erythroid differentiation. Finally, we show by both footprint analysis and functional assays that the ability of the G + C-rich region to increase alpha-globin promoter activity from a stably integrated alpha-globin gene is mediated by its multiple binding sites for the transcription factor Sp1.
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