ArticleThe Journal of clinical investigation1995
Enhanced G protein activation in immortalized lymphoblasts from patients with essential hypertension.
Article in The Journal of clinical investigation, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 3 of them syntheses that pooled it.
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Who cites it
31 citing papers in PubMed, 3 syntheses or guidelines pooled it, 180 citations in OpenAlex.
- Association of G-protein β3 subunit C825T polymorphism with essential hypertension: evidence from 63 729 subjects.Journal of human hypertension · 2017Pooled it
- Association between the G-protein β3 subunit C825T polymorphism with essential hypertension: a meta-analysis in Han Chinese population.Molecular biology reports · 2012Pooled it
- Association of α-adducin and G-protein β3 genetic polymorphisms with hypertension: a meta-analysis of Chinese populations.PloS one · 2011Pooled it
- The association between the GNB3 rs5443 C/C genotype and obesity phenotypes in Taiwanese individuals.BioMedicine · 2026Article
- Identification and validation of cholesterol metabolism-related gene GNB3 as prognosis biomarker for pancreatic adenocarcinoma.Discover oncology · 2025Article
- The Role of Single-Nucleotide Polymorphisms in Cholangiocarcinoma: A Systematic Review.Cancers · 2022Review
- cAMP guided his way: a life for G protein-mediated signal transduction and molecular pharmacology-tribute to Karl H. Jakobs.Naunyn-Schmiedeberg's archives of pharmacology · 2019Review
- Heterogeneity of B Cell Functions in Stroke-Related Risk, Prevention, Injury, and Repair.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2016Review
- Prediction of the Risk for Essential Hypertension among Carriers of C825T Genetic Polymorphism of G Protein β3 (GNB3) Gene.Biomarker insights · 2016Article
- Does the GNB3 C825T Polymorphism Influence Swimming Performance in Competitive Swimmers?Journal of human kinetics · 2015Article
- Is gnb3 c825t polymorphism associated with elite status of polish athletes?Biology of sport · 2014Article
- Antibodies in the pathogenesis of hypertension.BioMed research international · 2014Review
- Association of the G-protein β3 subunit gene polymorphism with the incidence of cardiovascular disease independent of hypertension: the Funagata study.Journal of human hypertension · 2013Article
- Association of the C825T polymorphism in the GNB3 gene with obesity and metabolic phenotypes in a Taiwanese population.Genes & nutrition · 2013Article
- Association of GNB3 C825T polymorphism with plasma electrolyte balance and susceptibility to hypertension.Genetics and molecular biology · 2011Article
- Inhibitory effect of somatostatin-14 on L-type voltage-gated calcium channels in cultured cone photoreceptors requires intracellular calcium.Journal of neurophysiology · 2009Article
- The GNB3 C825T polymorphism and depression among subjects with alcohol dependence.Journal of neural transmission (Vienna, Austria : 1996) · 2007Article
- Update on G-protein polymorphisms in hypertension.Current hypertension reports · 2006Review
- Studies of the association of the GNB3 825C>T polymorphism with components of the metabolic syndrome in white Danes.Diabetologia · 2006Article
- Quantification of G protein Gaalphas subunit splice variants in different human tissues and cells using pyrosequencing.Gene expression · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epstein-Barr virus-immortalized B lymphoblasts obtained from hypertensive patients with enhanced Na+/H+ exchanger activity (HT cells) proliferate distinctly faster upon serum stimulation than those from normotensive controls with low exchanger activity (NT cells) (Rosskopf, D., E. Frömter, and W. Siffert. 1993. J. Clin. Invest. 92:2553-2559). Stimulation with platelet-activating factor (PAF) as well caused an enhanced proliferation of HT cells. In analyzing possible differences in signal transduction between the immortalized NT and HT lymphoblasts, we observed that cell stimulation with PAF and somatostatin caused a twofold higher increase in [Ca2+]i in HT than in NT cell lines. This difference was completely abrogated by pertussis toxin (PTX) treatment. Furthermore, PAF-stimulated formation of inositol 1,4,5-trisphosphate (IP3) was twofold enhanced in HT cell lines. On the other hand, PAF receptor density and affinity, total cellular phospholipase C activity, expression of PTX-sensitive G proteins, and control binding of the stable GTP analogue, guanosine 5'-[gamma-thio]triphosphate (GTP gamma S), to membrane G proteins were not different in NT and HT cell lines. However, PAF- and mastoparan-stimulated binding of GTP gamma S to G proteins, which was fully PTX-sensitive, was 2.5-fold higher in HT than NT cell lines. These data suggest an enhanced receptor-mediated activation of PTX-sensitive G proteins despite unchanged receptor and G protein expression. Thus, this study not only suggests that enhanced signal transduction and cell proliferation are abnormalities in a certain group of patients with essential hypertension but also explains these findings as a result of an enhanced G protein activation in this common disorder.
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