ArticleGenetics1995
Genetic analysis of the fimbrin-actin binding interaction in Saccharomyces cerevisiae.
Article in Genetics, 1995. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- PLS3 missense variants affecting the actin-binding domains cause X-linked congenital diaphragmatic hernia and body-wall defects.American journal of human genetics · 2023Article
- The Dictyostelium discoideum FimA protein, unlike yeast and plant fimbrins, is regulated by calcium similar to mammalian plastins.Scientific reports · 2023Article
- The Calcium-Dependent Switch Helix of L-Plastin Regulates Actin Bundling.Scientific reports · 2017Article
- Actin filament bundling by fimbrin is important for endocytosis, cytokinesis, and polarization in fission yeast.The Journal of biological chemistry · 2011Article
- Arabidopsis FIMBRIN5, an actin bundling factor, is required for pollen germination and pollen tube growth.The Plant cell · 2010Article
- High-resolution cryo-EM structure of the F-actin-fimbrin/plastin ABD2 complex.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
- Suppressor mutations define two regions in the Cbp1 protein important for mitochondrial cytochrome b mRNA stability in Saccharomyces cerevisiae.Current genetics · 2003Article
- Interactions among a fimbrin, a capping protein, and an actin-depolymerizing factor in organization of the fission yeast actin cytoskeleton.Molecular biology of the cell · 2001Article
- An atomic model of actin filaments cross-linked by fimbrin and its implications for bundle assembly and function.The Journal of cell biology · 2001Article
- Generation of an isogenic collection of yeast actin mutants and identification of three interrelated phenotypes.Genetics · 2001Article
- Multiple functions for actin during filamentous growth of Saccharomyces cerevisiae.Molecular biology of the cell · 1998Article
- Amphiphysin I antisense oligonucleotides inhibit neurite outgrowth in cultured hippocampal neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Allele-specific suppression by formation of new protein-protein interactions in yeast.Genetics · 1997Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Yeast fimbrin is encoded by the SAC6 gene, mutations of which suppress temperature-sensitive mutations in the actin gene (ACT1). To examine the mechanism of suppression, we have sequenced 17 sac6 suppressor alleles, and found that they change nine different residues, all of which cluster in three regions of one of the two actin-binding domains of Sac6p. Two of these clusters occur in highly conserved regions (ABS1 and ABS3) that have been strongly implicated in the binding of related proteins to actin. The third cluster changes residues not previously implicated in the interaction with actin. As changes in any of nine different residues can suppress several different act1 alleles, it is likely that the suppressors restore the overall affinity, rather than specific lost interactions, between Sac6p and actin. Using mutagenesis, we have identified two mutations of the second actin-binding domain that can also suppress the act1 mutations of interest. This result suggests the two actin-binding domains of Sac6p interact with the same region of the actin molecule. However, differences in strength of suppression of temperature-sensitivity and sporulation indicate that the two actin-binding domains are distinct, and explain why second-domain mutations were not identified previously.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.